RRC ID 49703
Author D'Orazi FD, Zhao XF, Wong RO, Yoshimatsu T.
Title Mismatch of Synaptic Patterns between Neurons Produced in Regeneration and during Development of the Vertebrate Retina.
Journal Curr Biol
Abstract Stereotypic patterns of synaptic connections between neurons underlie the ability of the CNS to perform complex but circuit-specific information processing. Tremendous progress has been made toward advancing our understanding of how circuits are assembled during development, but whether the precision of this process can be recaptured after regeneration of neurons in the damaged CNS remains unclear. Here, we harnessed the endogenous regenerative capacity of the zebrafish retina to reconstruct the circuitry of neurons produced after damage. We tracked the input connectivity of identified bipolar cell (BC) types across stages of retinal development and after BC regeneration. We found that BCs of each type generate a unique and stereotypic wiring pattern with cone photoreceptors by gaining synapses with specific photoreceptor types over time. After selective ablation, the targeted BC types are rapidly reproduced and largely re-establish their characteristic morphological features. The regenerated population connects with appropriate photoreceptor types and establishes the original number of synaptic contacts. However, BC types that normally bias their connectivity in favor of red cones fail to precisely recapture this synaptic partner preference upon regeneration. Furthermore, regenerated BCs succeed in forming synaptic specializations at their axon terminals, but in excess of the usual number. Altogether, we find that regenerated BCs reinstate some, but not all, major features of their stereotypic wiring, suggesting that circuit patterns may be unable to regenerate with the same fidelity as in development.
Volume 26(17)
Pages 2268-79
Published 2016-9-12
DOI 10.1016/j.cub.2016.06.063
PII S0960-9822(16)30704-7
PMID 27524481
PMC PMC5534240
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / growth & development Animals, Genetically Modified / physiology Neurons / physiology* Regeneration* Retina / growth & development Retina / physiology* Retinal Cone Photoreceptor Cells / physiology* Synapses / physiology Zebrafish / genetics Zebrafish / growth & development Zebrafish / physiology*
IF 9.193
Times Cited 15
Resource
Zebrafish Tg(zfSWS1-5.5A:EGFP) Tg(zfSWS2-3.5A:EGFP)