Reference - Detail
| RRC ID | 49724 |
|---|---|
| Author | Aoki K, Kondo Y, Naoki H, Hiratsuka T, Itoh RE, Matsuda M. |
| Title | Propagating Wave of ERK Activation Orients Collective Cell Migration. |
| Journal | Dev Cell |
| Abstract |
The biophysical framework of collective cell migration has been extensively investigated in recent years; however, it remains elusive how chemical inputs from neighboring cells are integrated to coordinate the collective movement. Here, we provide evidence that propagation waves of extracellular signal-related kinase (ERK) mitogen-activated protein kinase activation determine the direction of the collective cell migration. A wound-healing assay of Mardin-Darby canine kidney (MDCK) epithelial cells revealed two distinct types of ERK activation wave, a "tidal wave" from the wound, and a self-organized "spontaneous wave" in regions distant from the wound. In both cases, MDCK cells collectively migrated against the direction of the ERK activation wave. The inhibition of ERK activation propagation suppressed collective cell migration. An ERK activation wave spatiotemporally controlled actomyosin contraction and cell density. Furthermore, an optogenetic ERK activation wave reproduced the collective cell migration. These data provide new mechanistic insight into how cells sense the direction of collective cell migration. |
| Volume | 43(3) |
| Pages | 305-317.e5 |
| Published | 2017-11-6 |
| DOI | 10.1016/j.devcel.2017.10.016 |
| PII | S1534-5807(17)30829-8 |
| PMID | 29112851 |
| MeSH | Actomyosin / metabolism Animals Cell Movement / physiology* Dogs Enzyme Activation Epithelial Cells / cytology* Kidney / metabolism MAP Kinase Signaling System / physiology* Mitogen-Activated Protein Kinases / metabolism* Phosphorylation Wound Healing / physiology |
| IF | 10.092 |
| Times Cited | 47 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 19 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pCMV-VSV-G-RSV-Rev (RDB04393) |
| Human and Animal Cells | MDCK(RCB0995) |