Reference - Detail
| RRC ID | 4972 |
|---|---|
| Author | Liu IF, Annamalai T, Sutherland JH, Tse-Dinh YC. |
| Title | Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex. |
| Journal | J Bacteriol |
| Abstract |
Escherichia coli expressing SOS-inducing mutant topoisomerase I was utilized to demonstrate that covalent protein-DNA complex accumulation results in oxidative damage. Hydroxyl radicals were detected following mutant topoisomerase induction. The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing. |
| Volume | 191(16) |
| Pages | 5315-9 |
| Published | 2009-8-1 |
| DOI | 10.1128/JB.00559-09 |
| PII | JB.00559-09 |
| PMID | 19525344 |
| PMC | PMC2725605 |
| MeSH | 2,2'-Dipyridyl / pharmacology Anti-Bacterial Agents / pharmacology Arabinose / pharmacology DNA / metabolism DNA Topoisomerases, Type I / genetics DNA Topoisomerases, Type I / metabolism DNA Topoisomerases, Type I / physiology* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / metabolism* Hydroxyl Radical / metabolism* Iron Chelating Agents / pharmacology Mutation Norfloxacin / pharmacology |
| IF | 3.006 |
| Times Cited | 18 |
| WOS Category | MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | Keio collection JW2514 |