RRC ID 4973
著者 Messing SA, Gabelli SB, Liu Q, Celesnik H, Belasco JG, Piñeiro SA, Amzel LM.
タイトル Structure and biological function of the RNA pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus.
ジャーナル Structure
Abstract Until recently, the mechanism of mRNA decay in bacteria was thought to be different from that of eukaryotes. This paradigm changed with the discovery that RppH (ORF176/NudH/YgdP), an Escherichia coli enzyme that belongs to the Nudix superfamily, is an RNA pyrophosphohydrolase that initiates mRNA decay by cleaving pyrophosphate from the 5'-triphosphate. Here we report the 1.9 Angstroms resolution structure of the Nudix hydrolase BdRppH from Bdellovibrio bacteriovorus, a bacterium that feeds on other Gram-negative bacteria. Based on the structure of the enzyme alone and in complex with GTP-Mg2+, we propose a mode of RNA binding similar to that of the nuclear decapping enzyme from Xenopus laevis, X29. In additional experiments, we show that BdRppH can indeed function in vitro and in vivo as an RNA pyrophosphohydrolase. These findings set the basis for the identification of possible decapping enzymes in other bacteria.
巻・号 17(3)
ページ 472-81
公開日 2009-3-11
DOI 10.1016/j.str.2008.12.022
PII S0969-2126(09)00075-6
PMID 19278661
PMC PMC2993163
MeSH Bacterial Proteins / chemistry* Bacterial Proteins / metabolism Bdellovibrio / enzymology* Catalysis Crystallography, X-Ray Dimerization Escherichia coli / genetics Escherichia coli / metabolism Models, Molecular Protein Conformation Pyrophosphatases / chemistry* Pyrophosphatases / metabolism RNA / metabolism* RNA Stability RNA, Bacterial / metabolism*
IF 4.945
引用数 20
原核生物(大腸菌) CV-25(DH5alpha) JW2798