Reference - Detail
RRC ID | 4980 |
---|---|
Author | Ninnis RL, Spall SK, Talbo GH, Truscott KN, Dougan DA. |
Title | Modification of PATase by L/F-transferase generates a ClpS-dependent N-end rule substrate in Escherichia coli. |
Journal | EMBO J |
Abstract |
The N-end rule pathway is conserved from bacteria to man and determines the half-life of a protein based on its N-terminal amino acid. In Escherichia coli, model substrates bearing an N-degron are recognised by ClpS and degraded by ClpAP in an ATP-dependent manner. Here, we report the isolation of 23 ClpS-interacting proteins from E. coli. Our data show that at least one of these interacting proteins--putrescine aminotransferase (PATase)--is post-translationally modified to generate a primary N-degron. Remarkably, the N-terminal modification of PATase is generated by a new specificity of leucyl/phenylalanyl-tRNA-protein transferase (LFTR), in which various combinations of primary destabilising residues (Leu and Phe) are attached to the N-terminal Met. This modification (of PATase), by LFTR, is essential not only for its recognition by ClpS, but also determines the stability of the protein in vivo. Thus, the N-end rule pathway, through the ClpAPS-mediated turnover of PATase may have an important function in putrescine homeostasis. In addition, we have identified a new element within the N-degron, which is required for substrate delivery to ClpA. |
Volume | 28(12) |
Pages | 1732-44 |
Published | 2009-6-17 |
DOI | 10.1038/emboj.2009.134 |
PII | emboj2009134 |
PMID | 19440203 |
PMC | PMC2699360 |
MeSH | Amino Acid Motifs Amino Acid Sequence Aminoacyltransferases / metabolism* Bacterial Outer Membrane Proteins / metabolism Carrier Proteins / chemistry Carrier Proteins / metabolism* Chromatography, Affinity Dipeptides / metabolism Endopeptidase Clp / chemistry Endopeptidase Clp / metabolism Escherichia coli / enzymology* Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* Hydrophobic and Hydrophilic Interactions Ligands Metabolic Networks and Pathways* Models, Biological Molecular Sequence Data Mutant Proteins / metabolism Protein Binding Protein Processing, Post-Translational* Substrate Specificity Transaminases / metabolism* |
IF | 9.889 |
Times Cited | 60 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
Resource | |
Prokaryotes E. coli | ME9062(BW25113) JW0866 JW0865 JW0427 JW0868 JW0797 JW5510 |