論文 - 詳細
| RRC ID | 50005 |
|---|---|
| 著者 | Kanda N, Ichikawa M, Ono A, Toyoda A, Fujiyama A, Abe J, Tsuchikane Y, Nishiyama T, Sekimoto H. |
| タイトル | CRISPR/Cas9-based knockouts reveal that CpRLP1 is a negative regulator of the sex pheromone PR-IP in the Closterium peracerosum-strigosum-littorale complex. |
| ジャーナル | Sci Rep |
| Abstract |
Heterothallic strains of the Closterium peracerosum-strigosum-littorale (C. psl.) complex have two sexes, mating-type plus (mt+) and mating-type minus (mt-). Conjugation between these two sexes is regulated by two sex pheromones, protoplast-release-inducing protein (PR-IP) and PR-IP Inducer, which are produced by mt+ and mt- cells, respectively. PR-IP mediates the release of protoplasts from mt- cells during mating. In this study, we examined the mechanism of action of CpRLP1 (receptor-like protein 1), which was previously identified in a cDNA microarray analysis as one of the PR-IP-inducible genes. Using CRISPR/Cas9 technology, we generated CpRLP1 knockout mutants in mt- cells of the C. psl. complex. When the knockout mt- cells were mixed with wild-type mt+ cells, conjugation was severely reduced. Many cells released protoplasts without pairing, suggesting a loss of synchronization between the two mating partners. Furthermore, the knockout mutants were hypersensitive to PR-IP. We conclude that CpRLP1 is a negative regulator of PR-IP that regulates the timing of protoplast release in conjugating C. psl. cells. As the first report of successful gene knockout in the class Charophyceae, this study provides a basis for research aimed at understanding the ancestral roles of genes that are indispensable for the development of land plants. |
| 巻・号 | 7(1) |
| ページ | 17873 |
| 公開日 | 2017-12-19 |
| DOI | 10.1038/s41598-017-18251-8 |
| PII | 10.1038/s41598-017-18251-8 |
| PMID | 29259295 |
| PMC | PMC5736609 |
| MeSH | Amino Acid Sequence Arabidopsis Proteins / genetics* Biological Phenomena / genetics CRISPR-Cas Systems / genetics CRISPR-Cas Systems / physiology* Carrier Proteins / genetics* Closterium / genetics Closterium / physiology* Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / physiology Gene Knockout Techniques / methods Protoplasts / physiology Reproduction / genetics Reproduction / physiology* Sex Attractants / genetics Sex Attractants / physiology* |
| IF | 3.998 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-67 NIES-68 |