論文 - 詳細
| RRC ID | 5007 |
|---|---|
| 著者 | Tominaga T, Hatakeyama Y. |
| タイトル | Development of innovative pediocin PA-1 by DNA shuffling among class IIa bacteriocins. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Pediocin PA-1 is a member of the class IIa bacteriocins, which show antimicrobial effects against lactic acid bacteria. To develop an improved version of pediocin PA-1, reciprocal chimeras between pediocin PA-1 and enterocin A, another class IIa bacteriocin, were constructed. Chimera EP, which consisted of the C-terminal half of pediocin PA-1 fused to the N-terminal half of enterocin A, showed increased activity against a strain of Leuconostoc lactis isolated from a sour-spoiled dairy product. To develop an even more effective version of this chimera, a DNA-shuffling library was constructed, wherein four specific regions within the N-terminal half of pediocin PA-1 were shuffled with the corresponding sequences from 10 other class IIa bacteriocins. Activity screening indicated that 63 out of 280 shuffled mutants had antimicrobial activity. A colony overlay activity assay showed that one of the mutants (designated B1) produced a >7.8-mm growth inhibition circle on L. lactis, whereas the parent pediocin PA-1 did not produce any circle. Furthermore, the active shuffled mutants showed increased activity against various species of Lactobacillus, Pediococcus, and Carnobacterium. Sequence analysis revealed that the active mutants had novel N-terminal sequences; in active mutant B1, for example, the parental pediocin PA-1 sequence (KYYGNGVTCGKHSC) was changed to TKYYGNGVSCTKSGC. These new and improved DNA-shuffled bacteriocins could prove useful as food additives for inhibiting sour spoilage of dairy products. |
| 巻・号 | 73(16) |
| ページ | 5292-9 |
| 公開日 | 2007-8-1 |
| DOI | 10.1128/AEM.00558-07 |
| PII | AEM.00558-07 |
| PMID | 17601819 |
| PMC | PMC1950993 |
| MeSH | Amino Acid Sequence Bacteriocins / genetics* Bacteriocins / metabolism DNA Shuffling / methods* Molecular Sequence Data Mutation Pediocins Pediococcus / genetics Pediococcus / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Reproducibility of Results Sequence Homology, Amino Acid |
| IF | 4.016 |
| 引用数 | 20 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM6123 JCM9695 JCM9698 JCM9694 JCM12225 JCM12535 JCM9697 JCM9696 JCM2026 JCM11052 |