RRC ID 5009
Author Cronin M, Knobel M, O'Connell-Motherway M, Fitzgerald GF, van Sinderen D.
Title Molecular dissection of a bifidobacterial replicon.
Journal Appl. Environ. Microbiol.
Abstract The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 10(6) transformants/mug DNA.
Volume 73(24)
Pages 7858-66
Published 2007-12
DOI 10.1128/AEM.01630-07
PII AEM.01630-07
PMID 17965208
PMC PMC2168138
MeSH Bifidobacterium / genetics* DNA Helicases / genetics DNA Helicases / metabolism DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli / genetics Genetic Vectors / genetics Molecular Sequence Data Plasmids / genetics* Protein Binding Replication Origin / genetics Replicon / genetics* Sequence Analysis, DNA Trans-Activators / genetics Trans-Activators / metabolism Transcription, Genetic Transformation, Bacterial
IF 3.633
Times Cited 22
General Microbes JCM5820