論文 - 詳細
| RRC ID | 5009 |
|---|---|
| 著者 | Cronin M, Knobel M, O'Connell-Motherway M, Fitzgerald GF, van Sinderen D. |
| タイトル | Molecular dissection of a bifidobacterial replicon. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 10(6) transformants/mug DNA. |
| 巻・号 | 73(24) |
| ページ | 7858-66 |
| 公開日 | 2007-12-1 |
| DOI | 10.1128/AEM.01630-07 |
| PII | AEM.01630-07 |
| PMID | 17965208 |
| PMC | PMC2168138 |
| MeSH | Bifidobacterium / genetics* DNA Helicases / genetics DNA Helicases / metabolism DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli / genetics Genetic Vectors / genetics Molecular Sequence Data Plasmids / genetics* Protein Binding Replication Origin / genetics Replicon / genetics* Sequence Analysis, DNA Trans-Activators / genetics Trans-Activators / metabolism Transcription, Genetic Transformation, Bacterial |
| IF | 4.016 |
| 引用数 | 26 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 43 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM5820 |