| RRC ID |
5009
|
| 著者 |
Cronin M, Knobel M, O'Connell-Motherway M, Fitzgerald GF, van Sinderen D.
|
| タイトル |
Molecular dissection of a bifidobacterial replicon.
|
| ジャーナル |
Appl Environ Microbiol
|
| Abstract |
The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 10(6) transformants/mug DNA.
|
| 巻・号 |
73(24)
|
| ページ |
7858-66
|
| 公開日 |
2007-12-1
|
| DOI |
10.1128/AEM.01630-07
|
| PII |
AEM.01630-07
|
| PMID |
17965208
|
| PMC |
PMC2168138
|
| MeSH |
Bifidobacterium / genetics*
DNA Helicases / genetics
DNA Helicases / metabolism
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Escherichia coli / genetics
Genetic Vectors / genetics
Molecular Sequence Data
Plasmids / genetics*
Protein Binding
Replication Origin / genetics
Replicon / genetics*
Sequence Analysis, DNA
Trans-Activators / genetics
Trans-Activators / metabolism
Transcription, Genetic
Transformation, Bacterial
|
| IF |
4.016
|
| 引用数 |
26
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM5820 |
