論文 - 詳細
| RRC ID | 50096 |
|---|---|
| 著者 | Kohzaki H, Asano M, Murakami Y. |
| タイトル | DNA replication machinery is required for development in Drosophila. |
| ジャーナル | Front Biosci (Landmark Ed) |
| Abstract |
In Drosophila, some factors involved in chromosome replication seem to be involved in gene amplification and endoreplication, which are actively utilized in particular tissue development, but direct evidence has not been shown. Therefore, we examined the effect of depletion of replication factors on these processes. First, we confirmed RNAi knockdown can be used for the depletion of replication factors by comparing the phenotypes of RNAi knockdown and deletion or point mutants of the components of DNA licensing factor, MCM2, MCM4 and Cdt1. Next, we found that tissue-specific RNAi knockdown of replication factors caused tissue-specific defects, probably due to defects in DNA replication. In particular, we found that depletion inhibited gene amplification of the chorion gene in follicle cells and endoreplication in salivary glands, showing that chromosomal DNA replication factors are required for these processes. Finally, using RNAi, we screened the genes for chromosomal DNA replication that affected tissue development. Interestingly, wing specific knockdown of Mcm10 induced wing formation defects. These results suggest that some components of chromosomal replication machinery are directly involved in tissue development. |
| 巻・号 | 23(3) |
| ページ | 493-505 |
| 公開日 | 2018-1-1 |
| DOI | 10.2741/4601 |
| PII | 4601 |
| PMID | 28930557 |
| MeSH | Animals Animals, Genetically Modified Cell Cycle Proteins / genetics DNA Replication / genetics* DNA-Binding Proteins / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Minichromosome Maintenance Complex Component 2 / genetics Minichromosome Maintenance Complex Component 4 / genetics Minichromosome Maintenance Proteins / genetics Mutation Organ Specificity / genetics RNA Interference* Wings, Animal / growth & development Wings, Animal / metabolism |
| IF | 2.747 |
| 引用数 | 2 |
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| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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