Reference - Detail
RRC ID | 50283 |
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Author | Pastor-Pareja JC, Xu T. |
Title | Shaping cells and organs in Drosophila by opposing roles of fat body-secreted Collagen IV and perlecan. |
Journal | Dev Cell |
Abstract |
Basement membranes (BMs) are resilient polymer structures that surround organs in all animals. Tissues, however, undergo extensive morphological changes during development. It is not known whether the assembly of BM components plays an active morphogenetic role. To study in vivo the biogenesis and assembly of Collagen IV, the main constituent of BMs, we used a GFP-based RNAi method (iGFPi) designed to knock down any GFP-trapped protein in Drosophila. We found with this method that Collagen IV is synthesized by the fat body, secreted to the hemolymph (insect blood), and continuously incorporated into the BMs of the larva. We also show that incorporation of Collagen IV determines organ shape, first by mechanically constricting cells and second through recruitment of Perlecan, which counters constriction by Collagen IV. Our results uncover incorporation of Collagen IV and Perlecan into BMs as a major determinant of organ shape and animal form. |
Volume | 21(2) |
Pages | 245-56 |
Published | 2011-8-16 |
DOI | 10.1016/j.devcel.2011.06.026 |
PII | S1534-5807(11)00260-7 |
PMID | 21839919 |
PMC | PMC4153364 |
MeSH | Animals Animals, Genetically Modified Basement Membrane / metabolism Cell Shape / physiology* Collagen Type IV / genetics Collagen Type IV / metabolism* Drosophila Drosophila Proteins / genetics Fat Body / cytology* Fat Body / metabolism* Fat Body / ultrastructure Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology Green Fluorescent Proteins / genetics Heparan Sulfate Proteoglycans / metabolism* Larva Microscopy, Confocal Microscopy, Electron, Transmission Models, Biological RNA Interference / physiology Wings, Animal / growth & development* Wings, Animal / ultrastructure |
IF | 10.092 |
Times Cited | 155 |
Resource | |
Drosophila | 6378R-1 16858R-3 |