RRC ID 50291
Author Lee J, Nonaka S, Takayama M, Ezura H.
Title Utilization of a Genome-Edited Tomato (Solanum lycopersicum) with High Gamma Aminobutyric Acid Content in Hybrid Breeding.
Journal J. Agric. Food Chem.
Abstract γ-Aminobutyric acid (GABA) is a nonproteogenic amino acid with health-promoting functions. Although tomato fruits have a relatively high GABA content compared with other crops, levels must be further increased to effectively confer the health-promoting functions. In this study, we evaluated the potential of the genome-edited tomato as a breeding material for producing high-GABA hybrid tomatoes. Hybrid lines were produced by crossing the genome-edited tomato with a pure line tomato cultivar, "Aichi First", and were evaluated for GABA accumulation and other fruit traits. The hybrid lines showed high GABA accumulation in the fruits, which was sufficiently high for expecting health-promoting functions and had minimal effects on other fruit traits, suggesting that the high GABA is a dominant trait and that the genome-edited tomato would be useful as a parental line of hybrid cultivars. These results also indicate that genome editing technology is useful for the rapid breeding of high-GABA hybrid tomato cultivars.
Volume 66(4)
Pages 963-971
Published 2018-1-31
DOI 10.1021/acs.jafc.7b05171
PMID 29314836
MeSH Alleles Amino Acid Sequence Amino Acids / analysis Carotenoids / analysis Gene Editing Genes, Plant Glutamate Decarboxylase / chemistry Glutamate Decarboxylase / genetics Hybridization, Genetic* Lycopersicon esculentum / chemistry* Lycopersicon esculentum / genetics* Lycopersicon esculentum / metabolism Mutation Plant Breeding / methods* Plants, Genetically Modified* / genetics gamma-Aminobutyric Acid / analysis* gamma-Aminobutyric Acid / metabolism
IF 3.412
Resource
Tomato TOMJPF00001 TOMJPF00003