RRC ID 50312
著者 Sasakura Y, Yoshida K, Treen N.
タイトル Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.
ジャーナル Methods Mol. Biol.
Abstract The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.
巻・号 1630
ページ 235-245
公開日 2017
DOI 10.1007/978-1-4939-7128-2_19
PMID 28643263
MeSH Animals Ciona intestinalis / genetics* Electroporation Gene Editing / methods* Gene Knockout Techniques Organ Specificity Transcription Activator-Like Effector Nucleases / metabolism
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int