Reference - Detail
| RRC ID | 50331 |
|---|---|
| Author | Ema M, Sogawa K, Watanabe N, Chujoh Y, Matsushita N, Gotoh O, Funae Y, Fujii-Kuriyama Y. |
| Title | cDNA cloning and structure of mouse putative Ah receptor. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene. |
| Volume | 184(1) |
| Pages | 246-53 |
| Published | 1992-4-15 |
| DOI | 10.1016/0006-291x(92)91185-s |
| PII | 0006-291X(92)91185-S |
| PMID | 1314586 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Line Chloramphenicol O-Acetyltransferase / genetics Chloramphenicol O-Acetyltransferase / metabolism Cloning, Molecular Gene Library Humans Methylcholanthrene / pharmacology Mice Molecular Sequence Data Oligonucleotide Probes Polychlorinated Dibenzodioxins / metabolism Polymerase Chain Reaction Receptors, Aryl Hydrocarbon Receptors, Drug / genetics* Recombinant Fusion Proteins / metabolism Restriction Mapping Sequence Homology, Nucleic Acid Transfection |
| IF | 2.985 |
| Times Cited | 370 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Mouse Ah receptor (RDB01364) |