| RRC ID |
50364
|
| Author |
Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S, Torii KU.
|
| Title |
Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair.
|
| Journal |
Nat Chem Biol
|
| Abstract |
The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch.
|
| Volume |
14(3)
|
| Pages |
299-305
|
| Published |
2018-3-1
|
| DOI |
10.1038/nchembio.2555
|
| PII |
nchembio.2555
|
| PMID |
29355850
|
| PMC |
PMC5812785
|
| MeSH |
Arabidopsis / chemistry
Arabidopsis / genetics
Arabidopsis Proteins / chemistry*
Crosses, Genetic
F-Box Proteins / chemistry*
Gene Expression Regulation, Plant*
Indoleacetic Acids / chemistry*
Kinetics
Mutation
Plant Growth Regulators
Plant Roots
Protein Binding
Protein Engineering
Receptors, Cell Surface / chemistry*
Seedlings
Signal Transduction
Transgenes
|
| IF |
12.587
|
| Times Cited |
25
|
| Resource |
| Yeast |
pEG202
pRS313 |
