RRC ID 50375
Author Woogeng IN, Kitakawa M.
Title The C-terminal extension domain of Saccharomyces cerevisiae MrpL32, a homolog of ribosomal protein L32, functions in trans to support mitochondrial translation.
Journal Genes Genet. Syst.
Abstract Mitochondrial ribosomal protein L32 (MrpL32) of Saccharomyces cerevisiae is homologous to the bacterial L32 ribosomal protein. MrpL32 carries an N-terminal mitochondrion-targeting sequence (MTS) and is about 60 amino acid residues longer at the C-terminus. Adding to its function as a leader sequence, the MTS of MrpL32 has been reported to regulate ribosome biogenesis through its processing by m-AAA protease. However, the function of the C-terminal extension (CE) remains totally unknown. Therefore, we constructed a series of C-terminally truncated mrpl32 (mrpl32ΔC) genes and expressed them in a Δmrpl32 mutant to examine their function. Interestingly, some MrpL32ΔC derivatives exhibited temperature-sensitive (ts) growth on medium with non-fermentable carbon sources. Furthermore, the CE domain of MrpL32, expressed separately from MrpL32ΔC, could rescue the ts phenotype of mutants by improving mitochondrial protein synthesis.
Volume 93(1)
Pages 21-24
Published 2018-7-13
DOI 10.1266/ggs.17-00023
PMID 29343666
MeSH 5' Untranslated Regions Culture Media Metalloendopeptidases / metabolism Mitochondria / metabolism* Mitochondrial Proteins / metabolism Mutation* Protein Biosynthesis Protein Domains Protein Sorting Signals Ribosomal Proteins / chemistry* Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Ribosomes / metabolism Saccharomyces cerevisiae / chemistry Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Temperature
IF 0.859
Yeast BY3072