Reference - Detail
| RRC ID | 50375 |
|---|---|
| Author | Woogeng IN, Kitakawa M. |
| Title | The C-terminal extension domain of Saccharomyces cerevisiae MrpL32, a homolog of ribosomal protein L32, functions in trans to support mitochondrial translation. |
| Journal | Genes Genet Syst |
| Abstract |
Mitochondrial ribosomal protein L32 (MrpL32) of Saccharomyces cerevisiae is homologous to the bacterial L32 ribosomal protein. MrpL32 carries an N-terminal mitochondrion-targeting sequence (MTS) and is about 60 amino acid residues longer at the C-terminus. Adding to its function as a leader sequence, the MTS of MrpL32 has been reported to regulate ribosome biogenesis through its processing by m-AAA protease. However, the function of the C-terminal extension (CE) remains totally unknown. Therefore, we constructed a series of C-terminally truncated mrpl32 (mrpl32ΔC) genes and expressed them in a Δmrpl32 mutant to examine their function. Interestingly, some MrpL32ΔC derivatives exhibited temperature-sensitive (ts) growth on medium with non-fermentable carbon sources. Furthermore, the CE domain of MrpL32, expressed separately from MrpL32ΔC, could rescue the ts phenotype of mutants by improving mitochondrial protein synthesis. |
| Volume | 93(1) |
| Pages | 21-24 |
| Published | 2018-7-13 |
| DOI | 10.1266/ggs.17-00023 |
| PMID | 29343666 |
| MeSH | 5' Untranslated Regions Culture Media Metalloendopeptidases / metabolism Mitochondria / metabolism* Mitochondrial Proteins / metabolism Mutation* Protein Biosynthesis Protein Domains Protein Sorting Signals Ribosomal Proteins / chemistry* Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Ribosomes / metabolism Saccharomyces cerevisiae / chemistry Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Temperature |
| IF | 0.917 |
| Times Cited | 0 |
| Resource | |
| Yeast | BY3072 |