論文 - 詳細
| RRC ID | 50388 |
|---|---|
| 著者 | Oguchi ME, Etoh K, Fukuda M. |
| タイトル | Rab20, a novel Rab small GTPase that negatively regulates neurite outgrowth of PC12 cells. |
| ジャーナル | Neurosci Lett |
| Abstract |
The Rab family small GTPases are key players in the membrane traffic that underlies various cellular phenomena. Neurite outgrowth, which is a prerequisite for neuronal network formation, also requires membrane traffic from the cell body to the tips of neurites. Although several Rabs have been shown to promote neurite outgrowth, very little is known about Rab involvement in the negative regulation of neurite outgrowth. Here we used nerve growth factor-stimulated PC12 cells to perform siRNA-based comprehensive knockdown screenings for Rabs that negatively regulate neurite outgrowth and succeeded in identifying Rab20 as a novel negative regulator of neurite outgrowth. Our findings showed that knockdown of endogenous Rab20 in PC12 cells promoted neurite outgrowth, whereas overexpression of active Rab20 inhibited it. We also found that the presence of Gly-64 and Cys-70, both of which are conserved only in the switch II region, a putative effector binding domain, of Rab20 is required for the inhibitory effect of Rab20 on neurite outgrowth. These findings indicated that active Rab20 suppresses neurite outgrowth of PC12 cells, possibly through interaction with an unidentified effector molecule that specifically recognizes certain amino acids in the switch II region of Rab20. |
| 巻・号 | 662 |
| ページ | 324-330 |
| 公開日 | 2018-1-1 |
| DOI | 10.1016/j.neulet.2017.10.056 |
| PII | S0304-3940(17)30882-0 |
| PMID | 29107708 |
| MeSH | Animals Neurites / metabolism* Neuronal Outgrowth / physiology* PC12 Cells Protein Transport / physiology Rats rab GTP-Binding Proteins / metabolism* |
| IF | 2.274 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-C1-mouse Rab20(G64A/C70Y) (RDB15730) pMRX-bsr-Myc-mouse Rab20-SR (RDB15731). |