論文 - 詳細
| RRC ID | 50390 |
|---|---|
| 著者 | Kuwana R, Ikejiri H, Yamamura S, Takamatsu H, Watabe K. |
| タイトル | Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis. |
| ジャーナル | Microbiology (Reading) |
| Abstract |
The sporulation-specific SpoVIF (YjcC) protein of Bacillus subtilis is essential for the development of heat-resistant spores. The GerE protein, the smallest member of the LuxR-FixJ family, contains a helix-turn-helix (HTH) motif and is involved in the expression of various sporulation-specific genes. In this study, the gene expression and protein composition of sporulating spoVIF-negative cells were analysed. CgeA, CotG and CotS, which are GerE-dependent coat proteins, were not expressed in the spoVIF-negative cells. Northern blotting showed that SpoVIF regulated the transcription of cgeA, cotG and cotS in a manner similar to that of GerE. In spoVIF-negative cells, gerE mRNA was transcribed normally, but immunoblot analysis using anti-GerE antiserum showed that the quantity of GerE protein was considerably less than that in wild-type controls. Using GFP (green fluorescent protein) fusion proteins, the localization of SpoVIF and GerE was observed by fluorescence microscopy. SpoVIF-GFP was detectable in the mother cell compartment, as was GerE-GFP. These results suggest that SpoVIF directly or indirectly controls the function of the GerE protein, and that SpoVIF is required for gene regulation during the latter stages of sporulation. |
| 巻・号 | 150(Pt 1) |
| ページ | 163-170 |
| 公開日 | 2004-1-1 |
| DOI | 10.1099/mic.0.26689-0 |
| PMID | 14702409 |
| MeSH | Bacillus subtilis / genetics* Bacillus subtilis / physiology* Bacterial Proteins / genetics* Bacterial Proteins / physiology* Base Sequence DNA, Bacterial / genetics Gene Expression Regulation, Bacterial Genes, Bacterial Membrane Proteins / genetics Membrane Proteins / physiology Plasmids / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spores, Bacterial / genetics Spores, Bacterial / physiology Transcription, Genetic |
| IF | 2.138 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS565 MBS566 |