RRC ID 50406
Author Sasakura Y.
Title The Enhancer Trap in Ciona.
Journal Adv Exp Med Biol
Abstract Enhancer trap is a famous application of transposons. This method is useful for the creation of marker transgenic lines that express a reporter gene in tissue- or organ-specific manner, characterization of enhancers in the genome, finding novel patterns of gene expression, and mutagenesis. In Ciona intestinalis, efficient enhancer traps with Minos and Sleeping beauty transposons have been reported. With the enhancer trap lines, the intronic enhancers regulating the expression of the Musashi gene, the compartment in the digestive tube, the presence of enhancers sensitive to the orientation of the gene that they regulate, and the functions of the Hox1 gene have been revealed. The enhancer trap lines generated with the transposon vectors are valuable resources for use as visual markers.
Volume 1029
Pages 121-129
Published 2018-1-1
DOI 10.1007/978-981-10-7545-2_11
PMID 29542085
MeSH Animals Animals, Genetically Modified / genetics* Ciona intestinalis / embryology Ciona intestinalis / genetics* Ciona intestinalis / growth & development DNA Transposable Elements / genetics* Digestive System / cytology Endoderm / metabolism Enhancer Elements, Genetic / genetics* Gene Expression Regulation, Developmental Genes, Homeobox Genes, Reporter Genetic Markers Gills / abnormalities Mutagenesis Mutagenesis, Insertional Transgenes*
IF 2.126
Times Cited 0
Resource
C.intestinalis / (O.japonicus) Wild C. int EJ[MiTSAdTPOG]15 EJ[MiTSAdTPOG]124 E[MiTSAdTPOG]19 E[MiTSAdTPOG]24 EJ[MiTSAdTPOG]75 EJ[MiTSAdTPOG]87 Mu[ISMiTSAdTPOG]1 E[MiCiTPOG]2