論文 - 詳細
| RRC ID | 50418 |
|---|---|
| 著者 | Chou HL, Tian L, Kumamaru T, Hamada S, Okita TW. |
| タイトル | Multifunctional RNA Binding Protein OsTudor-SN in Storage Protein mRNA Transport and Localization. |
| ジャーナル | Plant Physiol |
| Abstract |
The multifunctional RNA-binding protein Tudor-SN plays multiple roles in transcriptional and posttranscriptional processes due to its modular domain structure, consisting of four tandem Staphylococcus nuclease (SN)-like domains (4SN), followed by a carboxyl-terminal Tudor domain, followed by a fifth partial SN sequence (Tsn). In plants, it confers stress tolerance, is a component of stress granules and P-bodies, and may participate in stabilizing and localizing RNAs to specific subdomains of the cortical-endoplasmic reticulum in developing rice (Oryza sativa) endosperm. Here, we show that, in addition to the intact rice OsTudor-SN protein, the 4SN and Tsn modules exist as independent polypeptides, which collectively may coassemble to form a complex population of homodimer and heteroduplex species. The 4SN and Tsn modules exhibit different roles in RNA binding and as a protein scaffold for stress-associated proteins and RNA-binding proteins. Despite their distinct individual properties, mutations in both the 4SN and Tsn modules mislocalize storage protein mRNAs to the cortical endoplasmic reticulum. These results indicate that the two modular peptide regions of OsTudor-SN confer different cellular properties but cooperate in mRNA localization, a process linking its multiple functions in the nucleus and cytoplasm. |
| 巻・号 | 175(4) |
| ページ | 1608-1623 |
| 公開日 | 2017-12-1 |
| DOI | 10.1104/pp.17.01388 |
| PII | pp.17.01388 |
| PMID | 29084903 |
| PMC | PMC5717745 |
| MeSH | Gene Expression Regulation, Plant Models, Molecular Nuclear Proteins / genetics Nuclear Proteins / metabolism* Oryza / metabolism* Plant Proteins / genetics Plant Proteins / metabolism* Protein Conformation Protein Transport RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Plant / metabolism* |
| IF | 6.902 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| イネ | Induced mutation lines |