論文 - 詳細
| RRC ID | 50421 |
|---|---|
| 著者 | Hirai S, Hotta K, Kubo Y, Nishino A, Okabe S, Okamura Y, Okado H. |
| タイトル | AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
AMPA-type glutamate receptors (GluAs) mediate fast excitatory transmission in the vertebrate central nervous system (CNS), and their function has been extensively studied in the mature mammalian brain. However, GluA expression begins very early in developing embryos, suggesting that they may also have unidentified developmental roles. Here, we identify developmental roles for GluAs in the ascidian Ciona intestinalis Mammals express Ca2+-permeable GluAs (Ca-P GluAs) and Ca2+-impermeable GluAs (Ca-I GluAs) by combining subunits derived from four genes. In contrast, ascidians have a single gluA gene. Taking advantage of the simple genomic GluA organization in ascidians, we knocked down (KD) GluAs in Ciona and observed severe impairments in formation of the ocellus, a photoreceptive organ used during the swimming stage, and in resorption of the tail and body axis rotation during metamorphosis to the adult stage. These defects could be rescued by injection of KD-resistant GluAs. GluA KD phenotypes could also be reproduced by expressing a GluA mutant that dominantly inhibits glutamate-evoked currents. These results suggest that, in addition to their role in synaptic communication in mature animals, GluAs also have critical developmental functions. |
| 巻・号 | 114(15) |
| ページ | 3939-3944 |
| 公開日 | 2017-4-11 |
| DOI | 10.1073/pnas.1612943114 |
| PII | 1612943114 |
| PMID | 28348228 |
| PMC | PMC5393200 |
| MeSH | Amino Acid Substitution Animals Calcium / metabolism Ciona intestinalis / genetics Ciona intestinalis / growth & development* Ciona intestinalis / metabolism Female Gene Expression Regulation, Developmental Gene Knockdown Techniques Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Larva Male Morphogenesis Oocytes / physiology Receptors, Glutamate / genetics Receptors, Glutamate / metabolism* Sense Organs / growth & development* Sense Organs / metabolism Xenopus |
| IF | 9.412 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |