RRC ID 50432
Author Kurokawa Y, Nagai K, Huan PD, Shimazaki K, Qu H, Mori Y, Toda Y, Kuroha T, Hayashi N, Aiga S, Itoh JI, Yoshimura A, Sasaki-Sekimoto Y, Ohta H, Shimojima M, Malik AI, Pedersen O, Colmer TD, Ashikari M.
Title Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance.
Journal New Phytol
Abstract Floods impede gas (O2 and CO2 ) exchange between plants and the environment. A mechanism to enhance plant gas exchange under water comprises gas films on hydrophobic leaves, but the genetic regulation of this mechanism is unknown. We used a rice mutant (dripping wet leaf 7, drp7) which does not retain gas films on leaves, and its wild-type (Kinmaze), in gene discovery for this trait. Gene complementation was tested in transgenic lines. Functional properties of leaves as related to gas film retention and underwater photosynthesis were evaluated. Leaf Gas Film 1 (LGF1) was identified as the gene determining leaf gas films. LGF1 regulates C30 primary alcohol synthesis, which is necessary for abundant epicuticular wax platelets, leaf hydrophobicity and gas films on submerged leaves. This trait enhanced underwater photosynthesis 8.2-fold and contributes to submergence tolerance. Gene function was verified by a complementation test of LGF1 expressed in the drp7 mutant background, which restored C30 primary alcohol synthesis, wax platelet abundance, leaf hydrophobicity, gas film retention, and underwater photosynthesis. The discovery of LGF1 provides an opportunity to better understand variation amongst rice genotypes for gas film retention ability and to target various alleles in breeding for improved submergence tolerance for yield stability in flood-prone areas.
Volume 218(4)
Pages 1558-1569
Published 2018-6-1
DOI 10.1111/nph.15070
PMID 29498045
MeSH Adaptation, Physiological* Base Sequence Biosynthetic Pathways Floods* Gases / metabolism* Genes, Plant* Genetic Complementation Test Hydrophobic and Hydrophilic Interactions* Mutation / genetics Oryza / genetics* Oryza / physiology Photosynthesis Plant Epidermis / metabolism Plant Leaves / physiology* Plant Proteins / genetics Plant Proteins / metabolism Waxes / metabolism*
IF 8.512
Times Cited 22
Rice Induced mutation lines