Reference - Detail
|Author||Dhingra A, Alexander D, Reyes-Reveles J, Sharp R, Boesze-Battaglia K.|
|Title||Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina.|
|Journal||Adv Exp Med Biol|
Microtubule-associated protein 1 light chain 3 (MAP1LC3), a human homologue of yeast Atg8, is an essential component of autophagy. LC3 plays a critical role in hybrid degradation pathways in which some but not all components of autophagy are coupled with phagocytosis in a process known as LC3-associated phagocytosis (LAP). LC3 exists as three highly homologous isoforms in human (LC3A, LC3B, and LC3C) with two of these (LC3A and LC3B) in mouse. LC3B predominated in both fetal and adult human retinal pigment epithelium (RPE) relative to LC3A and LC3C, while in mouse RPE and neural retina, LC3A and LC3B were expressed at approximately equivalent levels. In situ hybridization studies localized LC3A and LC3B transcripts in the retina and RPE. LC3B protein was detected in C57Bl6/J RPE and retinal lysates and was absent in the LC3BKO mouse.
|MeSH||Alternative Splicing Animals Autophagy Cell Line Eye Proteins / analysis* Gene Expression Regulation Humans Immunoblotting In Situ Hybridization Mice Mice, Inbred C57BL Mice, Knockout Microtubule-Associated Proteins / analysis* Microtubule-Associated Proteins / deficiency Microtubule-Associated Proteins / genetics Protein Isoforms / analysis RNA, Messenger / biosynthesis RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Retina / chemistry* Retina / ultrastructure Retinal Pigment Epithelium / chemistry Retinal Pigment Epithelium / ultrastructure|