RRC ID 50499
Author Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
Title A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
Journal Biochem Biophys Res Commun
Abstract In our previous study, we produced a microfluidic device (MFD) which successfully maintained spermatogenesis for over 6 months in mouse testis tissues loaded in the device. In the present study, we developed a new MFD, a monolayer device (ML-D) with a barrier structure consisting of pillars and slits, which is simpler in design and easier to make. This ML-D was also effective for inducing mouse spermatogenesis and maintained it for a longer period than the conventional culture method. In addition, we devised a way of introducing sample tissue into the device during its production, just before bonding the upper layer of polydimethylsiloxane (PDMS) and bottom glass slide. The tissue can obtain nutrients horizontally from the medium running beside it and oxygen vertically from above through PDMS. In addition, the glass slide set at the bottom improved the visibility of the sample tissue with an inverted microscope. When we took photos of cultured tissue of the Acr-Gfp transgenic mouse testis in ML-D sequentially every day, morphological changes of the acrosome during spermiogenesis were successfully recorded. The ML-D is simple in design and useful for culturing testis tissue for inducing and maintaining spermatogenesis with clearer visibility. Due to the new method of sample loading, tissues other than testis should also be applicable.
Volume 500(4)
Pages 885-891
Published 2018-6-12
DOI 10.1016/j.bbrc.2018.04.180
PII S0006-291X(18)30973-2
PMID 29705697
MeSH Animals Dimethylpolysiloxanes / chemistry Equipment Design / instrumentation* Gene Expression Genes, Reporter Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Lab-On-A-Chip Devices* Male Mice Mice, Transgenic Spermatogenesis / genetics* Spermatozoa / growth & development Spermatozoa / metabolism Spermatozoa / ultrastructure* Testis / cytology* Testis / growth & development Testis / metabolism Tissue Culture Techniques
IF 2.985
Times Cited 9
Mice RBRC00445 RBRC00886