RRC ID 50523
Author Tsutsui H, Higashijima S, Miyawaki A, Okamura Y.
Title Visualizing voltage dynamics in zebrafish heart.
Journal J. Physiol. (Lond.)
Abstract The zebrafish heart provides a useful vertebrate cardiovascular model with outstanding advantages, including genetic manipulatability, optical accessibility and rapid development. In addition, an emerging topic in cardiotoxicity assay and drug discovery is its use in phenotype-based chemical screening. Here, we report a technique that allows non-invasive voltage mapping in beating heart using a genetically encoded probe for transmembrane potential. Application of the anti-allergy compound astemizole resulted in aberrant propagation of excitation, which accounted for a lack of ventricular contraction. This optical method will provide new opportunities in broad areas of physiological, developmental and pharmacological cardiovascular research.
Volume 588(Pt 12)
Pages 2017-21
Published 2010-6-15
DOI 10.1113/jphysiol.2010.189126
PII jphysiol.2010.189126
PMID 20421282
PMC PMC2911208
MeSH Action Potentials Animals Animals, Genetically Modified Anti-Allergic Agents / toxicity Astemizole / toxicity Biosensing Techniques Cardiac Myosins / genetics Dose-Response Relationship, Drug Ether-A-Go-Go Potassium Channels / antagonists & inhibitors Ether-A-Go-Go Potassium Channels / metabolism Excitation Contraction Coupling / drug effects Fluorescence Recovery After Photobleaching Heart Rate / drug effects Kinetics Luminescent Proteins / biosynthesis Luminescent Proteins / genetics Microscopy, Fluorescence Myocytes, Cardiac / drug effects Myocytes, Cardiac / metabolism* Myosin Light Chains / genetics Promoter Regions, Genetic Zebrafish / genetics Zebrafish / metabolism* Zebrafish Proteins / antagonists & inhibitors Zebrafish Proteins / metabolism
IF 4.984
Times Cited 35
WOS Category PHYSIOLOGY NEUROSCIENCES
Resource
DNA material ptol2/cmlc2-mermaid (RDB15780).