| RRC ID |
5052
|
| Author |
Zhang L, Xu Z, Patel BKC.
|
| Title |
Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
An aerobic bacterium, designated strain E1HC-02(T), was isolated from the decaying leaf litter of a slash pine forest located in southeast Queensland, Australia. Cells of strain E1HC-02(T) were short irregular rods (0.5-1.0 x 0.2-0.4 microm) which stained Gram-positive and possessed a cell-wall ultrastructure which appeared to be made of protein subunits. The novel strain grew optimally in 1 % trypticase soy broth (TSB) at 25 degrees C and at a pH of 9.1. Strain E1HC-02(T) metabolized a range of carbohydrates, organic acids and amino acids. The G+C content of the DNA was 71+/-1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis of strain E1HC-02(T) showed that it was a member of the family Microbacteriaceae, phylum Actinobacteria. The cell wall contained a type B2beta peptidoglycan, the dominant cellular fatty acid was 18 : 1omega7c and the major hydroxy fatty acid was 2-OH 14 : 0. The major menaquinones were MK-8 (76 %) and MK-7 (24 %) and the glycolipids present were disphosphatidylglycerol, phosphatidylglycerol and three unidentified phospholipids. The chemotaxonomic properties of strain E1HC-02(T) were distinctly different to all of the 17 genera of the family Microbacteriaceae and hence strain E1HC-02(T) is designated as representing a novel species of a new genus, Frondicola australicus gen. nov., sp. nov. The type strain of the type species is E1HC-02(T) (=JCM 13598(T)=DSM 17894(T)).
|
| Volume |
57(Pt 6)
|
| Pages |
1177-1182
|
| Published |
2007-6-1
|
| DOI |
10.1099/ijs.0.64560-0
|
| PMID |
17551025
|
| MeSH |
Actinomycetales / classification*
Actinomycetales / genetics
Actinomycetales / isolation & purification*
Actinomycetales / physiology
Aerobiosis
Australia
Base Composition
Carbohydrate Metabolism
Cell Wall / chemistry
Cell Wall / ultrastructure
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Genes, rRNA
Glycolipids / analysis
Hydrogen-Ion Concentration
Microscopy, Electron, Transmission
Molecular Sequence Data
Nucleic Acid Denaturation
Nucleic Acid Hybridization
Peptidoglycan / analysis
Phylogeny
Plant Leaves / microbiology
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Soil Microbiology*
Temperature
Trees
Vitamin K 2 / analysis
|
| IF |
2.415
|
| Times Cited |
20
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM13598 |
