論文 - 詳細
| RRC ID | 50531 |
|---|---|
| 著者 | Nagai N, Yamada S, Kawasaki J, Koyanagi E, Saijo S, Kaji H, Nishizawa M, Nakazawa T, Abe T. |
| タイトル | Pharmacokinetic and Safety Evaluation of a Transscleral Sustained Unoprostone Release Device in Monkey Eyes. |
| ジャーナル | Invest Ophthalmol Vis Sci |
| Abstract |
Purpose:We evaluate the ocular tissue distribution and retinal toxicity of unoprostone (UNO) during 12 months, after transscleral sustained-UNO administration using a drug delivery device in monkey eyes. Methods:The device consisted of a reservoir, controlled-release cover, and a drug formulation of photopolymerized polyethylene glycol dimethacrylate. Six mg UNO was loaded into the device (length, 17 mm; width, 4.4 mm; height, 1 mm). The concentrations of M1, a primary metabolite of UNO, in the retina, choroid, vitreous, lens, aqueous humor, iris, ciliary body, and plasma were determined by liquid chromatography-tandem mass spectrometry at 3, 6, and 12 months after implantation. Retinal toxicity was evaluated by electroretinography (ERG), optical coherence tomography (OCT), and IOP at preimplantation, and at 6, 9, and 12 months after implantation. Focal ERGs were performed at 9 and 12 months after implantation. Results:M1 was detected in the choroid and retina with maximum peaks of 243.2 and 8.41 ng/g at 6 months, respectively. M1 in the ciliary body and iris was detected with maximum peaks of 7.66 and 10.4 ng/g at 6 and 12 months, respectively. Less than 1 ng/mL or ng/g of M1 was detected in the aqueous humor, vitreous, and lens. No changes were observed in retinal function as assessed by ERG, IOP, or macula thickness and retinal histology by OCT examinations during the 12-month period. No differences in focal ERG amplitudes, especially in the macula, were observed. Conclusions:The device provided intraocular sustained delivery of UNO for 12 months without producing severe retinal toxicity. |
| 巻・号 | 59(2) |
| ページ | 644-652 |
| 公開日 | 2018-2-1 |
| DOI | 10.1167/iovs.17-22429 |
| PII | 2671735 |
| PMID | 29392308 |
| MeSH | Animals Antihypertensive Agents / pharmacokinetics* Antihypertensive Agents / toxicity Chromatography, Liquid Delayed-Action Preparations Dinoprost / analogs & derivatives* Dinoprost / pharmacokinetics Dinoprost / toxicity Drug Delivery Systems* Drug Evaluation, Preclinical Electroretinography Intraocular Pressure / drug effects Macaca Macaca fascicularis Methacrylates / chemistry Polyethylene Glycols / chemistry Retina / drug effects Retina / physiology Sclera / metabolism Tandem Mass Spectrometry Tissue Distribution Tomography, Optical Coherence |
| IF | 3.47 |
| 引用数 | 5 |
| リソース情報 | |
| ニホンザル | |