RRC ID 50546
Author Osugi T, Sasakura Y, Satake H.
Title The nervous system of the adult ascidian Ciona intestinalis Type A (Ciona robusta): Insights from transgenic animal models.
Journal PLoS One
Abstract The nervous system of ascidians is an excellent model system to provide insights into the evolutionary process of the chordate nervous system due to their phylogenetic positions as the sister group of vertebrates. However, the entire nervous system of adult ascidians has yet to be functionally and anatomically investigated. In this study, we have revealed the whole dorsal and siphon nervous system of the transgenic adult ascidian of Ciona intestinalis Type A (Ciona robusta) in which a Kaede reporter gene is expressed in a pan-neuronal fashion. The fluorescent signal of Kaede revealed the innervation patterns and distribution of neurons in the nervous system of Ciona. Precise microscopic observation demonstrated the clear innervation of the anterior and posterior main nerves to eight and six lobes of the oral and atrial siphons, respectively. Moreover, visceral nerves, previously identified as unpaired nerves, were found to be paired; one nerve was derived from the posterior end of the cerebral ganglion and the other from the right posterior nerve. This study further revealed the full trajectory of the dorsal strand plexus and paired visceral nerves on either side from the cerebral ganglion to the ovary, and precise innervation between the cerebral ganglion and the peripheral organs including the gonoduct, cupular organ, rectum and ovary. The differential innervation patterns of visceral nerves and the dorsal strand plexus indicate that the peripheral organs including the ovary undergo various neural regulations. Collectively, the present anatomical analysis revealed the major innervation of the dorsal and siphon nervous systems of adult Ciona.
Volume 12(6)
Pages e0180227
Published 2017-6-26
DOI 10.1371/journal.pone.0180227
PII PONE-D-17-12023
PMID 28651020
PMC PMC5484526
MeSH Animals Animals, Genetically Modified Biological Evolution Ciona intestinalis / anatomy & histology* Ciona intestinalis / genetics Ciona intestinalis / metabolism Female Genes, Reporter Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Nervous System / anatomy & histology* Nervous System / metabolism Ovary / innervation Recombinant Proteins / genetics Recombinant Proteins / metabolism
IF 2.74
Times Cited 10
C.intestinalis / (O.japonicus) Wild C. int Tg[MiCiPC2K]2 Tg[MiCiPC2K]3