| RRC ID |
5057
|
| 著者 |
Hua NP, Kanekiyo A, Fujikura K, Yasuda H, Naganuma T.
|
| タイトル |
Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two Gram-positive, rod-shaped, moderately halophilic bacteria were isolated from a deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll, Japan. These bacteria, strains IS-Hb4(T) and IS-Hb7(T), were spore-forming and non-motile. They were able to grow at temperatures as low as 9 degrees C and hydrostatic pressures up to 30 MPa. Based on high sequence similarity of their 16S rRNA genes to those of type strains of the genus Halobacillus, from 96.4 % (strain IS-Hb7(T) to Halobacillus halophilus NCIMB 9251(T)) to 99.4 % (strain IS-Hb4(T) to Halobacillus dabanensis D-8(T)), the strains were shown to belong to this genus. DNA-DNA relatedness values of 49.5 % and 1.0-33.0 %, respectively, were determined between strains IS-Hb4(T) and IS-Hb7(T) and between these strains and other Halobacillus type strains. Both strains showed the major menaquinone MK7 and L-orn-D-Asp cell-wall peptidoglycan type. Straight-chain C(16 : 0), unsaturated C(16 : 1)omega7c alcohol and C(18 : 1)omega7c and cyclopropane C(19 : 0) cyc fatty acids were predominant in both strains. The DNA G+C contents of IS-Hb4(T) and IS-Hb7(T) were respectively 43.3 and 42.1 mol%. Physiological and biochemical analyses combined with DNA-DNA hybridization results allowed us to place strains IS-Hb4(T) (=JCM 14154(T)=DSM 18394(T)) and IS-Hb7(T) (=JCM 14155(T)=DSM 18393(T)) in the genus Halobacillus as the respective type strains of the novel species Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov.
|
| 巻・号 |
57(Pt 6)
|
| ページ |
1243-1249
|
| 公開日 |
2007-6-1
|
| DOI |
10.1099/ijs.0.64817-0
|
| PMID |
17551037
|
| MeSH |
Bacillaceae / classification*
Bacillaceae / cytology
Bacillaceae / isolation & purification*
Bacillaceae / physiology
Bacterial Typing Techniques
Base Composition
Cold Temperature
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Genes, rRNA
Hydrostatic Pressure
Japan
Locomotion
Microscopy, Phase-Contrast
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
Quinones / analysis
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Spores, Bacterial
|
| IF |
2.415
|
| 引用数 |
29
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM14154
JCM14155
JCM11546 |
