論文 - 詳細
| RRC ID | 50575 |
|---|---|
| 著者 | Ansai S, Kinoshita M. |
| タイトル | Genome Editing of Medaka. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka. |
| 巻・号 | 1630 |
| ページ | 175-188 |
| 公開日 | 2017-1-1 |
| DOI | 10.1007/978-1-4939-7128-2_15 |
| PMID | 28643259 |
| MeSH | Animals Electrophoresis, Microchip / methods* Gene Editing / methods* Gene Knockout Techniques Genetic Vectors / administration & dosage Models, Animal Oryzias / genetics* Plasmids / genetics |
| リソース情報 | |
| メダカ | database |