RRC ID 50578
著者 Bodine TJ, Evangelista MA, Chang HT, Ayoub CA, Samuel BS, Sucgang R, Zechiedrich L.
タイトル Escherichia coli DNA ligase B may mitigate damage from oxidative stress.
ジャーナル PLoS One
Abstract Escherichia coli encodes two DNA ligases, ligase A, which is essential under normal laboratory growth conditions, and ligase B, which is not. Here we report potential functions of ligase B. We found that across the entire Enterobacteriaceae family, ligase B is highly conserved in both amino acid identity and synteny with genes associated with oxidative stress. Deletion of ligB sensitized E. coli to specific DNA damaging agents and antibiotics resulted in a weak mutator phenotype, and decreased biofilm formation. Overexpression of ligB caused a dramatic extension of lag phase that eventually resumed normal growth. The ligase function of ligase B was not required to mediate the extended lag phase, as overexpression of a ligase-deficient ligB mutant also blocked growth. Overexpression of ligB during logarithmic growth caused an immediate block of cell growth and DNA replication, and death of about half of cells. These data support a potential role for ligase B in the base excision repair pathway or the mismatch repair pathway.
巻・号 12(7)
ページ e0180800
公開日 2017-7-11
DOI 10.1371/journal.pone.0180800
PII PONE-D-16-43810
PMID 28700629
PMC PMC5507437
MeSH DNA Damage / genetics DNA Ligases / genetics DNA Ligases / metabolism* DNA Replication / genetics DNA Replication / physiology Enterobacteriaceae / genetics Enterobacteriaceae / metabolism Escherichia coli / enzymology* Escherichia coli / metabolism* Oxidative Stress / genetics Oxidative Stress / physiology
IF 2.74
引用数 1
リソース情報
原核生物(大腸菌) JW3622-KC