論文 - 詳細
| RRC ID | 50612 |
|---|---|
| 著者 | Osawa R, Kamide T, Satoh Y, Kawano Y, Ohtsu I, Dairi T. |
| タイトル | Heterologous and High Production of Ergothioneine in Escherichia coli. |
| ジャーナル | J Agric Food Chem |
| Abstract |
Ergothioneine (ERG) is a histidine-derived thiol compound suggested to function as an antioxidant and cytoprotectant in humans. Therefore, experimental trials have been conducted applying ERG from mushrooms in dietary supplements and as a cosmetic additive. However, this method of producing ERG is expensive; therefore, alternative methods for ERG supply are required. Five Mycobacterium smegmatis genes, egtABCDE, have been confirmed to be responsible for ERG biosynthesis. This enabled us to develop practical fermentative ERG production by microorganisms. In this study, we carried out heterologous and high-level production of ERG in Escherichia coli using the egt genes from M. smegmatis. By high production of each of the Egt enzymes and elimination of bottlenecks in the substrate supply, we succeeded in constructing a production system that yielded 24 mg/L (104 μM) secreted ERG. |
| 巻・号 | 66(5) |
| ページ | 1191-1196 |
| 公開日 | 2018-2-7 |
| DOI | 10.1021/acs.jafc.7b04924 |
| PMID | 29276826 |
| MeSH | Antioxidants Cytoprotection Ergothioneine / biosynthesis* Escherichia coli / genetics Escherichia coli / metabolism* Fermentation Gene Transfer Techniques Mycobacterium smegmatis / growth & development Recombinant Proteins / biosynthesis Transfection |
| IF | 3.571 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |
| 一般微生物 | JCM 6386 |