| RRC ID |
50622
|
| Author |
Otani K, Niwa Y, Suzuki T, Sato N, Sasazawa Y, Dohmae N, Simizu S.
|
| Title |
Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling.
|
| Volume |
498(3)
|
| Pages |
466-472
|
| Published |
2018-4-6
|
| DOI |
10.1016/j.bbrc.2018.02.210
|
| PII |
S0006-291X(18)30466-2
|
| PMID |
29501745
|
| MeSH |
Amino Acid Sequence
Cell Line, Tumor
Granulocytes / cytology*
Granulocytes / metabolism
HEK293 Cells
Humans
Leukopoiesis*
Mannose / analysis
Mannose / metabolism*
Receptors, Granulocyte Colony-Stimulating Factor / chemistry
Receptors, Granulocyte Colony-Stimulating Factor / metabolism*
Signal Transduction
|
| IF |
2.985
|
| Times Cited |
3
|
| Resource |
| DNA material |
CSII-CMV-MCS-IRES2-Bsd (RDB04385). |
