Reference - Detail
| RRC ID | 50622 |
|---|---|
| Author | Otani K, Niwa Y, Suzuki T, Sato N, Sasazawa Y, Dohmae N, Simizu S. |
| Title | Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling. |
| Volume | 498(3) |
| Pages | 466-472 |
| Published | 2018-4-6 |
| DOI | 10.1016/j.bbrc.2018.02.210 |
| PII | S0006-291X(18)30466-2 |
| PMID | 29501745 |
| MeSH | Amino Acid Sequence Cell Line, Tumor Granulocytes / cytology* Granulocytes / metabolism HEK293 Cells Humans Leukopoiesis* Mannose / analysis Mannose / metabolism* Receptors, Granulocyte Colony-Stimulating Factor / chemistry Receptors, Granulocyte Colony-Stimulating Factor / metabolism* Signal Transduction |
| IF | 2.985 |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CSII-CMV-MCS-IRES2-Bsd (RDB04385). |