論文 - 詳細
| RRC ID | 50657 |
|---|---|
| 著者 | Gundlapally SR, Garcia-Pichel F. |
| タイトル | Description of Deinococcus oregonensis sp. nov., from biological soil crusts in the Southwestern arid lands of the United States of America. |
| ジャーナル | Arch Microbiol |
| Abstract |
Biological soil crusts are distinct habitats, harbor unique prokaryotic diversity and gave an impetus to isolate novel species. In the present study, a pink-pigmented bacterium, (OR316-6T), was isolated from biological soil crusts using oligotrophic BG11-PGY medium. Strain OR316-6T was Gram-positive, short rods, non-motile and non-spore forming. Cells were positive for catalase, oxidase and β-galactosidase and negative for most of the enzymatic activities. The major fatty acids present were C16:0, C17:0, and C16:1ω7c and contained MK-8 and MK-10 as the predominant menaquinones. The cell wall peptidoglycan was of A3β variant with L-ornithine as the diamino acid. Based on the above characteristics, strain OR316-6T was assigned to the genus Deinococcus. The phylogenetic analysis indicated that strain OR316-6T was closely related to D. aquatilis DSM 23025T with a 16S rRNA gene similarity of 99.3 % and clustered with a bootstrap value of 100 %. DNA-DNA similarity between strain OR316-6T and D. aquatilis DSM 23025T was 37.0 % indicating that strain OR316-6T was a novel species. Further, DNA fingerprinting of stains OR316-6T and D. aquatilis DSM 23035T demonstrated that both strains were related to each other with a similarity coefficient of only 0.32 and supported the species status to strain OR316-6T. In addition, phenotypic characteristics distinguished strain OR316-6T from D. aquatilis DSM 23025T. Based on the cumulative differences, strain OR316-16T exhibited with its closely related species, it was identified as a novel species and proposed the name Deinococcus oregonensis sp. nov. The type strain is D. oregonensis sp. nov. (OR316-6T = JCM 13503T = DSM 17762T). |
| 巻・号 | 199(1) |
| ページ | 69-76 |
| 公開日 | 2017-1-1 |
| DOI | 10.1007/s00203-016-1273-x |
| PII | 10.1007/s00203-016-1273-x |
| PMID | 27515516 |
| MeSH | Bacterial Typing Techniques Cell Wall / chemistry Cell Wall / metabolism DNA, Bacterial / genetics Deinococcus / classification Deinococcus / genetics Deinococcus / isolation & purification* Deinococcus / metabolism Fatty Acids / chemistry Fatty Acids / metabolism Molecular Sequence Data Peptidoglycan / metabolism Phylogeny RNA, Ribosomal, 16S / genetics Soil Microbiology* Southwestern United States beta-Galactosidase / genetics beta-Galactosidase / metabolism |
| IF | 1.884 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 13503 |