RRC ID 50673
Author Sugiyama Y, Katoh T, Honda Y, Gotoh A, Ashida H, Kurihara S, Yamamoto K, Katayama T.
Title Application study of 1,2-α-l-fucosynthase: introduction of Fucα1-2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide.
Journal Biosci. Biotechnol. Biochem.
Abstract We have recently generated a highly efficient 1,2-α-l-fucosynthase (BbAfcA N423H mutant) by protein engineering of 1,2-α-l-fucosidase from Bifidobacterium bifidum JCM 1254. This synthase could specifically introduce H-antigens (Fucα1-2Gal) into the non-reducing ends of oligosaccharides and in O-linked glycans in mucin glycoprotein. In the present study, we show an extended application of the engineered 1,2-α-l-fucosynthase by demonstrating its ability to insert Fuc residues into N- and O-glycans in fetuin glycoproteins, GM1 ganglioside, and a plant-derived xyloglucan nonasaccharide. This application study broadens the feasibility of this novel H-antigen synthesis technique in functional glycomics.
Volume 81(2)
Pages 283-291
Published 2017-2
DOI 10.1080/09168451.2016.1254532
PMID 27832720
MeSH Asialoglycoproteins / metabolism Bifidobacterium / enzymology Disaccharides / chemistry* Disaccharides / metabolism* Fetuins / metabolism Fucose / chemistry* Fucose / metabolism Gangliosides / chemistry* Gangliosides / metabolism Glucans / chemistry* Glucans / metabolism Glycolipids / chemistry Glycolipids / metabolism Mutation Oligosaccharides / chemistry* Oligosaccharides / metabolism Plants / chemistry Protein Engineering Xylans / chemistry* Xylans / metabolism alpha-L-Fucosidase / genetics alpha-L-Fucosidase / metabolism*
IF 1.255
Resource
General Microbes JCM 1254