論文 - 詳細
| RRC ID | 50673 |
|---|---|
| 著者 | Sugiyama Y, Katoh T, Honda Y, Gotoh A, Ashida H, Kurihara S, Yamamoto K, Katayama T. |
| タイトル | Application study of 1,2-α-l-fucosynthase: introduction of Fucα1-2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
We have recently generated a highly efficient 1,2-α-l-fucosynthase (BbAfcA N423H mutant) by protein engineering of 1,2-α-l-fucosidase from Bifidobacterium bifidum JCM 1254. This synthase could specifically introduce H-antigens (Fucα1-2Gal) into the non-reducing ends of oligosaccharides and in O-linked glycans in mucin glycoprotein. In the present study, we show an extended application of the engineered 1,2-α-l-fucosynthase by demonstrating its ability to insert Fuc residues into N- and O-glycans in fetuin glycoproteins, GM1 ganglioside, and a plant-derived xyloglucan nonasaccharide. This application study broadens the feasibility of this novel H-antigen synthesis technique in functional glycomics. |
| 巻・号 | 81(2) |
| ページ | 283-291 |
| 公開日 | 2017-2-1 |
| DOI | 10.1080/09168451.2016.1254532 |
| PMID | 27832720 |
| MeSH | Asialoglycoproteins / metabolism Bifidobacterium / enzymology Disaccharides / chemistry* Disaccharides / metabolism* Fetuins / metabolism Fucose / chemistry* Fucose / metabolism Gangliosides / chemistry* Gangliosides / metabolism Glucans / chemistry* Glucans / metabolism Glycolipids / chemistry Glycolipids / metabolism Mutation Oligosaccharides / chemistry* Oligosaccharides / metabolism Plants / chemistry Protein Engineering Xylans / chemistry* Xylans / metabolism alpha-L-Fucosidase / genetics alpha-L-Fucosidase / metabolism* |
| IF | 1.516 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1254 |