| RRC ID |
50673
|
| 著者 |
Sugiyama Y, Katoh T, Honda Y, Gotoh A, Ashida H, Kurihara S, Yamamoto K, Katayama T.
|
| タイトル |
Application study of 1,2-α-l-fucosynthase: introduction of Fucα1-2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
We have recently generated a highly efficient 1,2-α-l-fucosynthase (BbAfcA N423H mutant) by protein engineering of 1,2-α-l-fucosidase from Bifidobacterium bifidum JCM 1254. This synthase could specifically introduce H-antigens (Fucα1-2Gal) into the non-reducing ends of oligosaccharides and in O-linked glycans in mucin glycoprotein. In the present study, we show an extended application of the engineered 1,2-α-l-fucosynthase by demonstrating its ability to insert Fuc residues into N- and O-glycans in fetuin glycoproteins, GM1 ganglioside, and a plant-derived xyloglucan nonasaccharide. This application study broadens the feasibility of this novel H-antigen synthesis technique in functional glycomics.
|
| 巻・号 |
81(2)
|
| ページ |
283-291
|
| 公開日 |
2017-2-1
|
| DOI |
10.1080/09168451.2016.1254532
|
| PMID |
27832720
|
| MeSH |
Asialoglycoproteins / metabolism
Bifidobacterium / enzymology
Disaccharides / chemistry*
Disaccharides / metabolism*
Fetuins / metabolism
Fucose / chemistry*
Fucose / metabolism
Gangliosides / chemistry*
Gangliosides / metabolism
Glucans / chemistry*
Glucans / metabolism
Glycolipids / chemistry
Glycolipids / metabolism
Mutation
Oligosaccharides / chemistry*
Oligosaccharides / metabolism
Plants / chemistry
Protein Engineering
Xylans / chemistry*
Xylans / metabolism
alpha-L-Fucosidase / genetics
alpha-L-Fucosidase / metabolism*
|
| IF |
1.516
|
| 引用数 |
9
|
| リソース情報 |
| 一般微生物 |
JCM 1254 |
