RRC ID 50678
Author Kim J, Park C, Imlay JA, Park W.
Title Lineage-specific SoxR-mediated Regulation of an Endoribonuclease Protects Non-enteric Bacteria from Redox-active Compounds.
Journal J Biol Chem
Abstract Bacteria use redox-sensitive transcription factors to coordinate responses to redox stress. The [2Fe-2S] cluster-containing transcription factor SoxR is particularly tuned to protect cells against redox-active compounds (RACs). In enteric bacteria, SoxR is paired with a second transcription factor, SoxS, that activates downstream effectors. However, SoxS is absent in non-enteric bacteria, raising questions as to how SoxR functions. Here, we first show that SoxR of Acinetobacter oleivorans displayed similar activation profiles in response to RACs as did its homolog from Escherichia coli but controlled a different set of target genes, including sinE, which encodes an endoribonuclease. Expression, gel mobility shift, and mutational analyses indicated that sinE is a direct target of SoxR. Redox potentials and permeability of RACs determined optimal sinE induction. Bioinformatics suggested that only a few γ- and β-proteobacteria might have SoxR-regulated sinE Purified SinE, in the presence of Mg2+ ions, degrades rRNAs, thus inhibiting protein synthesis. Similarly, pretreatment of cells with RACs demonstrated a role for SinE in promoting persistence in the presence of antibiotics that inhibit protein synthesis. Our data improve our understanding of the physiology of soil microorganisms by suggesting that both non-enteric SoxR and its target SinE play protective roles in the presence of RACs and antibiotics.
Volume 292(1)
Pages 121-133
Published 2017-1-6
DOI 10.1074/jbc.M116.757500
PII M116.757500
PMID 27895125
PMC PMC5217672
MeSH Acinetobacter / drug effects Acinetobacter / metabolism* Anti-Bacterial Agents / pharmacology* Bacterial Proteins / metabolism* Cell Lineage Endoribonucleases / metabolism* Escherichia coli / drug effects Escherichia coli / metabolism Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial / drug effects* Gene Expression Regulation, Enzymologic / drug effects* Iron-Sulfur Proteins / metabolism Oxidation-Reduction / drug effects* Protein Biosynthesis / drug effects Trans-Activators Transcription Factors / metabolism* Transcription, Genetic
IF 4.238
Times Cited 10
Resource
General Microbes JCM 16667