RRC ID 5068
Author Gutiérrez MC, Castillo AM, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa A.
Title Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline Lake Shangmatala in Inner Mongolia, China.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Strain SH-6(T) was isolated from the sediment of Lake Shangmatala, a saline lake in Inner Mongolia (China). Cells were pleomorphic. The organism was neutrophilic and required at least 2.5 M (15 %) NaCl, but not MgCl(2), for growth; optimal growth occurred at 4.3 M (25 %) NaCl. The G+C content of its DNA was 63.1 mol%. 16S rRNA gene sequence analysis revealed that strain SH-6(T) is a member of the family Halobacteriaceae, but there was a low level of similarity with other members of this family. Highest sequence similarity (94.6 %) was obtained with the 16S rRNA genes of the type strains of Natronolimnobius innermongolicus and Natronolimnobius baerhuensis. Polar lipid analyses revealed that strain SH-6(T) contains phosphatidylglycerol and phosphatidylglyceromethylphosphate, derived from both C(20)C(20) and C(20)C(25) glycerol diethers together with the glycolipid S(2)-DGD-1. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain SH-6(T) is thus considered to represent a novel species in a new genus within the family Halobacteriaceae, order Halobacteriales, for which the name Halopiger xanaduensis gen. nov., sp. nov. is proposed. The type strain of Halopiger xanaduensis is SH-6(T) (=CECT 7173(T)=CGMCC 1.6379(T)=JCM 14033(T)).
Volume 57(Pt 7)
Pages 1402-7
Published 2007-7
DOI 10.1099/ijs.0.65001-0
PII 57/7/1402
PMID 17625165
MeSH Base Composition China DNA, Archaeal / chemistry DNA, Archaeal / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Genes, rRNA Geologic Sediments / microbiology* Halobacteriaceae / chemistry Halobacteriaceae / classification* Halobacteriaceae / isolation & purification* Halobacteriaceae / physiology Magnesium Chloride / metabolism Microscopy, Phase-Contrast Molecular Sequence Data Phospholipids / analysis Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism
IF 1.932
Times Cited 30
WOS Category MICROBIOLOGY
Resource
General Microbes JCM14033