論文 - 詳細
| RRC ID | 50759 |
|---|---|
| 著者 | Chen S, Xu Y, Ke LX. |
| タイトル | Halorubrum trueperi sp. nov., a halophilic archaeon isolated from a salt mine. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel, extremely halophilic archaeon, strain Y73T, was isolated from a salt mine in Yunnan, China. Colonies formed on solid medium were circular (2-3 mm in diameter), smooth, orange, glistening and convex (~1 mm in elevation). Cells were Gram-stain-negative, non-motile and pleomorphic. Mg2+ was required for growth (optimum at 0.05 M). Optimal growth was observed at 20 % (w/v) NaCl, 42-45 °C and pH 7.5-8.5 under aerobic conditions. 16S rRNA gene sequence comparison showed that strain Y73T was closely related to Halorubrum halophilum B8T (similarity: 98.1 %), Halorubrum lipolyticum 9-3T (97.9 %) and Halorubrum saccharovorum JCM 8865T (97.6 %). Levels of rpoB' gene sequence similarity between strain Y73T and H. halophilum B8T, H. lipolyticum 9-3T and H. saccharovorum JCM 8865T were 93.6, 93.8 and 94.7 %, respectively. DNA-DNA relatedness between strain Y73T and H. halophilum B8T was 38.7±0.5 %, while that between strain Y73T and H. saccharovorum JCM 8865T was 31.0±0.9 % . The DNA G+C content of strain Y73T was 61.9 mol%. The major polar lipids of strain Y73T were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate as phospholipids, and sulfated diglycosyl diether-1 as glycolipid. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain Y73T represents a novel species, for which the name Halorubrum trueperi sp. nov is proposed. The type strain is Y73T (=CGMCC 1.15503T=JCM 31271T). |
| 巻・号 | 67(5) |
| ページ | 1564-1570 |
| 公開日 | 2017-5-1 |
| DOI | 10.1099/ijsem.0.001762 |
| PMID | 28032536 |
| MeSH | Base Composition China DNA, Archaeal / genetics Glycolipids / chemistry Halorubrum / classification* Halorubrum / genetics Halorubrum / isolation & purification Mining* Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride |
| IF | 2.415 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31271 JCM 8865 |