RRC ID 50812
Author Takehana Y, Umekita M, Hatano M, Kato C, Sawa R, Igarashi M.
Title Fradiamine A, a new siderophore from the deep-sea actinomycete Streptomyces fradiae MM456M-mF7.
Journal J. Antibiot.
Abstract New bioactive substances were identified from several marine actinomycetes strains by LC-HRESI-MS based non-targeted metabolomics. A new siderophore and its derivative, named fradiamines A and B, were isolated from the extract of the deep-sea actinomycetes Streptomyces fradiae MM456M-mF7 by Diaion CHP-20P, Sephadex LH-20 column chromatography and HPLC. Fradiamine A was a new compound, but fradiamine B was previously patented as a sweetness enhancer. Their structures were determined by NMR and LC-HRESI-MS/MS analysis. Fradiamines A and B contained two alkyl amines asymmetrically bonded to citrate, a type of structure derived from actinomycetes and other bacteria and rarely observed in siderophores. Fradiamines A and B showed moderate antibiotic activity against Clostridium difficile with IC50 values of 32 and 8 μg ml-1, respectively.
Volume 70(5)
Pages 611-615
Published 2017-5
DOI 10.1038/ja.2017.26
PII ja201726
PMID 28246378
MeSH Acetates / chemistry Acetates / isolation & purification Acetates / pharmacology* Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / isolation & purification Anti-Bacterial Agents / pharmacology* Chromatography, Liquid Clostridium difficile / drug effects* Diamines / chemistry Diamines / isolation & purification Diamines / pharmacology* Inhibitory Concentration 50 Magnetic Resonance Spectroscopy Mass Spectrometry Siderophores / chemistry Siderophores / isolation & purification Siderophores / pharmacology* Streptomyces / metabolism*
IF 2.446
Times Cited 10
Resource
General Microbes JCM 1386 JCM 1391 JCM 1380 JCM 1292 JCM 1427 JCM 1298