論文 - 詳細
| RRC ID | 50836 |
|---|---|
| 著者 | Watanabe M, Fukiya S, Yokota A. |
| タイトル | Comprehensive evaluation of the bactericidal activities of free bile acids in the large intestine of humans and rodents. |
| ジャーナル | J Lipid Res |
| Abstract |
In addition to functioning as detergents that aid digestion of dietary lipids in the intestine, some bile acids have been shown to exhibit antimicrobial activity. However, detailed information on the bactericidal activities of the diverse molecular species of bile acid in humans and rodents is largely unknown. Here, we investigated the toxicity of 14 typical human and rodent free bile acids (FBAs) by monitoring intracellular pH, membrane integrity, and viability of a human intestinal bacterium, Bifidobacterium breve Japan Collection of Microorganisms (JCM) 1192T, upon exposure to these FBAs. Of all FBAs evaluated, deoxycholic acid (DCA) and chenodeoxycholic acid displayed the highest toxicities. Nine FBAs common to humans and rodents demonstrated that α-hydroxy-type bile acids are more toxic than their oxo-derivatives and β-hydroxy-type epimers. In five rodent-specific FBAs, β-muricholic acid and hyodeoxycholic acid showed comparable toxicities at a level close to DCA. Similar trends were observed for the membrane-damaging effects and bactericidal activities to Blautia coccoides JCM 1395T and Bacteroides thetaiotaomicron DSM 2079T, commonly represented in the human and rodent gut microbiota. These findings will help us to determine the fundamental properties of FBAs and better understand the role of FBAs in the regulation of gut microbiota composition. |
| 巻・号 | 58(6) |
| ページ | 1143-1152 |
| 公開日 | 2017-6-1 |
| DOI | 10.1194/jlr.M075143 |
| PII | S0022-2275(20)31006-3 |
| PMID | 28404640 |
| PMC | PMC5454512 |
| MeSH | Animals Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / metabolism Anti-Bacterial Agents / pharmacology* Bacteroidaceae / cytology Bacteroidaceae / drug effects* Bacteroidaceae / physiology Bifidobacterium breve / cytology Bifidobacterium breve / drug effects* Bifidobacterium breve / physiology Bile Acids and Salts / chemistry Bile Acids and Salts / metabolism Bile Acids and Salts / pharmacology* Cell Membrane / drug effects Humans Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Intestine, Large / metabolism* Intracellular Space / drug effects Mice Microbial Viability / drug effects |
| IF | 4.483 |
| 引用数 | 20 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1192 JCM 1395 |