RRC ID 50851
著者 Kobatake E, Nakagawa H, Seki T, Miyazaki T.
タイトル Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress.
ジャーナル PLoS One
Abstract Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH2-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells.
巻・号 12(5)
ページ e0177106
公開日 2017-5-11
DOI 10.1371/journal.pone.0177106
PII PONE-D-16-49319
PMID 28493927
PMC PMC5426657
MeSH Animals Antioxidant Response Elements Cell Proliferation Cells, Cultured Fibroblasts / cytology Fibroblasts / metabolism* Fibroblasts / microbiology* JNK Mitogen-Activated Protein Kinases / metabolism Lactobacillus gasseri / metabolism* MAP Kinase Signaling System Mice NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism NIH 3T3 Cells Oxidative Stress* Probiotics / metabolism* Signal Transduction
IF 2.74
引用数 18
リソース情報
一般微生物 JCM 1131