| RRC ID |
50899
|
| 著者 |
Rasooli M, Naghoni A, Amoozegar MA, Mirfeizi L, Moshtaghi Nikou M, Shahzadeh Fazeli SA, Minegishi H, Ventosa A.
|
| タイトル |
Natrinema soli sp. nov., a novel halophilic archaeon isolated from a hypersaline wetland.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
An extremely halophilic archaeon, designated strain 5-3T, was isolated from a soil sample of Meighan wetland in Iran. Strain 5-3T was strictly aerobic, catalase-positive and oxidase-negative. Cells were Gram-stain-negative, non-motile and ovoid. Colonies of strain 5-3T were cream-coloured. The isolate showed optimum growth at 4.0 M NaCl, 40 °C and pH 7.0. The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two unknown phospholipids and three glycolipids (including one that was chromatographically identical to S2-DGD). The major respiratory quinone was menaquinone MK-8. The G+C content of the genomic DNA was 61.5 mol%. The closest relative was Natrinema salaciae JCM 17869T with 97.3 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and rpoB' gene sequences indicated that strain 5-3T is a member of the genus Natrinema in the family Natrialbaceae and forms a distinct cluster. On the basis of phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, a novel species of the family Natrialbaceae, Natrinema soli sp. nov., is proposed. The type strain is 5-3T (=IBRC-M 11063T=LMG 29247T).
|
| 巻・号 |
67(7)
|
| ページ |
2142-2147
|
| 公開日 |
2017-7-1
|
| DOI |
10.1099/ijsem.0.001909
|
| PMID |
28650314
|
| MeSH |
DNA, Archaeal / genetics
Genes, Archaeal
Glycolipids / chemistry
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification
Iran
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Salinity
Sequence Analysis, DNA
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
Wetlands*
|
| IF |
2.415
|
| 引用数 |
4
|
| リソース情報 |
| 一般微生物 |
JCM 13890 |
