| RRC ID |
50914
|
| 著者 |
Minegishi H, Enomoto S, Echigo A, Shimane Y, Kondo Y, Inoma A, Kamekura M, Takai K, Itoh T, Ohkuma M, Ihara K, Takahashi-Ando N, Fukushima Y, Ishii S, Yoshida Y, Usami R.
|
| タイトル |
Salinarchaeum chitinilyticum sp. nov., a chitin-degrading haloarchaeon isolated from commercial salt.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two chitin-degrading halophilic archaeal strains, MC-74T and MC-23, were isolated from commercial salt samples. Cells were motile, rod-shaped and stained Gram-negative. Colonies were vermillion-pigmented. Strains MC-74T and MC-23 were able to grow with 1.5-5.1 M NaCl (optimum, 2.6-3.1 M) at pH 6.0-10.0 (optimum, pH 7.0) and at 20-50 °C (optimum, 40 °C). The orthologous 16S rRNA gene sequence similarity between the two strains was 99.8 %, and the closest phylogenetic relative was Salinarchaeum laminariae JCM 17267T with 99.3-99.5 % similarity. The level of DNA-DNA relatedness between the two strains was 93 and 94 % (reciprocally), and those between the two strains and Salinarchaeumlaminariae JCM 17267T were 35-36 % and 38-39 % (reciprocally). The polar lipids of both strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Glycolipids were not detected. Based on the phenotypic and phylogenetic analyses, the strains represent a novel species of the genus Salinarchaeum, for which the name Salinarchaeum chitinilyticum sp. nov. is proposed. The type strain is MC-74T (=JCM 19597T=KCTC 4262T), isolated from solar salt produced in France. Strain MC-23, isolated from a commercial solar salt sample produced in China, is an additional strain of the species.
|
| 巻・号 |
67(7)
|
| ページ |
2274-2278
|
| 公開日 |
2017-7-1
|
| DOI |
10.1099/ijsem.0.001941
|
| PMID |
28671534
|
| MeSH |
China
Chitin / metabolism
DNA, Archaeal / genetics
France
Glycolipids / chemistry
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification
Neisseriaceae / genetics
Nucleic Acid Hybridization
Phosphatidylglycerols / chemistry
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / analysis*
|
| IF |
2.415
|
| 引用数 |
2
|
| リソース情報 |
| 一般微生物 |
JCM 19597
JCM 17267
JCM 8978
JCM 8877
JCM 8865 |
