RRC ID 50934
Author Briegel A, Oikonomou CM, Chang YW, Kjær A, Huang AN, Kim KW, Ghosal D, Nguyen HH, Kenny D, Ogorzalek Loo RR, Gunsalus RP, Jensen GJ.
Title Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis.
Journal EMBO Rep
Abstract Archaeal swimming motility is driven by archaella: rotary motors attached to long extracellular filaments. The structure of these motors, and particularly how they are anchored in the absence of a peptidoglycan cell wall, is unknown. Here, we use electron cryotomography to visualize the archaellar basal body in vivo in Thermococcus kodakaraensis KOD1. Compared to the homologous bacterial type IV pilus (T4P), we observe structural similarities as well as several unique features. While the position of the cytoplasmic ATPase appears conserved, it is not braced by linkages that extend upward through the cell envelope as in the T4P, but rather by cytoplasmic components that attach it to a large conical frustum up to 500 nm in diameter at its base. In addition to anchoring the lophotrichous bundle of archaella, the conical frustum associates with chemosensory arrays and ribosome-excluding material and may function as a polar organizing center for the coccoid cells.
Volume 18(9)
Pages 1660-1670
Published 2017-9-1
DOI 10.15252/embr.201744070
PII embr.201744070
PMID 28729461
PMC PMC5579351
MeSH Adenosine Triphosphatases / metabolism Archaeal Proteins / metabolism Cell Membrane / metabolism Cell Membrane / ultrastructure Cell Surface Extensions / physiology Cell Surface Extensions / ultrastructure* Cryoelectron Microscopy Cytoplasm / physiology Cytoplasm / ultrastructure* Flagella / physiology Flagella / ultrastructure Thermococcus / cytology Thermococcus / physiology* Thermococcus / ultrastructure*
IF 8.383
Times Cited 15
Resource
General Microbes JCM 12380