RRC ID 50935
Author Zachariah S, Das SK.
Title Idiomarina andamanensis sp. nov., an alkalitolerant bacterium isolated from Andaman Sea water.
Journal Antonie Van Leeuwenhoek
Abstract Two closely related aerobic, Gram-negative rod shaped bacteria (strain W5T and W3) were isolated from Andaman Sea. Heterotrophic growth on marine agar was observed at 15-45 °C and pH 6-10. Strain W5T showed maximum 16S rRNA sequence similarity of 99.58% with Idiomarina marina JCM 15083T. DNA fingerprinting analysis by ERIC-REP PCR, PFGE and MLSA revealed differences in banding patterns, also DNA-DNA hybridization values were well below 70% confirming W5T to be a new species. DNA G+C content was 46.7 mol%. Major fatty acids were iso-C15:0, iso-C17:0, iso-C17:1 ω9c, iso-C13:0 3OH, iso-C11:0 3OH and C16:0. Polar lipids included phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) diphosphatidylglycerol (DPG) phospholipid (PL) two aminolipids (AL) and two unidentified lipids (L1-2). Q-8 is the predominant ubiquinone. On the basis of polyphasic taxonomic study, strain W5T is considered to be representative of a new species of the genus Idiomarina, for which the name Idiomarina andamanensis sp. nov. is being proposed. The type strain W5T (= LMG 29773T = JCM 31645T) was isolated from Andaman Sea.
Volume 110(12)
Pages 1581-1592
Published 2017-12-1
DOI 10.1007/s10482-017-0908-5
PII 10.1007/s10482-017-0908-5
PMID 28730371
MeSH Bacterial Typing Techniques Base Composition Gammaproteobacteria / chemistry Gammaproteobacteria / classification* Gammaproteobacteria / genetics* Gammaproteobacteria / isolation & purification Metabolomics / methods Multilocus Sequence Typing Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Water Microbiology*
IF 2.674
Times Cited 2
General Microbes JCM 15533 JCM 15534 JCM 15083 JCM 15084 JCM 19499 JCM 31645