論文 - 詳細
| RRC ID | 50968 |
|---|---|
| 著者 | Katoh T, Maeshibu T, Kikkawa KI, Gotoh A, Tomabechi Y, Nakamura M, Liao WH, Yamaguchi M, Ashida H, Yamamoto K, Katayama T. |
| タイトル | Identification and characterization of a sulfoglycosidase from Bifidobacterium bifidum implicated in mucin glycan utilization. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Human gut symbiont bifidobacteria possess carbohydrate-degrading enzymes that act on the O-linked glycans of intestinal mucins to utilize those carbohydrates as carbon sources. However, our knowledge about mucin type O-glycan degradation by bifidobacteria remains fragmentary, especially regarding how they decompose sulfated glycans, which are abundantly found in mucin sugar-chains. Here, we examined the abilities of several Bifidobacterium strains to degrade a sulfated glycan substrate and identified a 6-sulfo-β-d-N-acetylglucosaminidase, also termed sulfoglycosidase, encoded by bbhII from Bifidobacterium bifidum JCM 7004. A recombinant BbhII protein showed a substrate preference toward 6-sulfated and 3,4-disulfated N-acetylglucosamines over non-sulfated and 3-sulfated N-acetylglucosamines. The purified BbhII directly released 6-sulfated N-acetylglucosamine from porcine gastric mucin and the expression of bbhII was moderately induced in the presence of mucin. This de-capping activity may promote utilization of sulfated glycans of mucin by other bacteria including bifidobacteria, thereby establishing the symbiotic relationship between human and gut microbes. |
| 巻・号 | 81(10) |
| ページ | 2018-2027 |
| 公開日 | 2017-10-1 |
| DOI | 10.1080/09168451.2017.1361810 |
| PMID | 28814130 |
| MeSH | Acetylglucosaminidase / chemistry Acetylglucosaminidase / genetics Acetylglucosaminidase / metabolism* Amino Acid Sequence Bifidobacterium bifidum / enzymology* Bifidobacterium bifidum / genetics Bifidobacterium bifidum / metabolism Gene Expression Regulation, Bacterial Mucins / metabolism* Polysaccharides / metabolism* |
| IF | 1.516 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 7004 JCM 1254 |