RRC ID 510
Author Tsuchida K, Jouni ZE, Gardetto J, Kobayashi Y, Tabunoki H, Azuma M, Sugiyama H, Takada N, Maekawa H, Banno Y, Fujii H, Iwano H, Wells MA.
Title Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori.
Journal J. Insect Physiol.
Abstract Carotenoids play important and diverse roles in insects. Recently, we purified and partially characterized a carotenoid-binding protein (CBP) from the wild type of Bombyx mori. In this report, we utilized immunoblotting, ELISA and immunocytochemistry to further characterize and localize the expression of CBP in the larval midgut and silk gland obtained from the wild type and four naturally occurring mutants linked to carotenoids transport. CBP was expressed throughout the 5th stadium, with highest expressions on days 4-5 in the silk gland and days 3-5 in the midgut. Immunoblotting analyses demonstrated the presence of CBP along the middle part of the midgut. Microscopic immunocytochemistry demonstrated that the 33 kDa CBP was uniformly expressed along the brush border of columnar cells in the epithelium of the midgut typifying its function in aiding absorption of dietary carotenoids. Similarly, CBP was highly expressed along the distal membrane of the middle part of the silk gland demonstrating its function in uptake of carotenoids from lipophorin. When the middle silk glands and midguts of the four mutants were incubated with rabbit anti-CBP antibody, only proteins of the Y-gene dominant mutants cross reacted with the antibody further accentuating the hypothesis that the CBP is a Y-gene dependent protein.
Volume 50(4)
Pages 363-72
Published 2004-4
DOI 10.1016/j.jinsphys.2004.02.006
PII S0022191004000265
PMID 15081829
MeSH Animals Bombyx / genetics Bombyx / metabolism* Carotenoids / metabolism* Carrier Proteins / biosynthesis* Carrier Proteins / genetics* Carrier Proteins / metabolism Exocrine Glands / metabolism Gastrointestinal Tract / metabolism Gastrointestinal Tract / ultrastructure Gene Expression Genes, Insect Immunoblotting Immunohistochemistry Insect Proteins / genetics Insect Proteins / metabolism* Larva / genetics Larva / metabolism Microvilli / metabolism Microvilli / ultrastructure Mutation
IF 2.733
Times Cited 18
Silkworms Y strain I strain ?