RRC ID 51080
著者 Brankovics B, van Dam P, Rep M, de Hoog GS, J van der Lee TA, Waalwijk C, van Diepeningen AD.
タイトル Mitochondrial genomes reveal recombination in the presumed asexual Fusarium oxysporum species complex.
ジャーナル BMC Genomics
Abstract BACKGROUND:The Fusarium oxysporum species complex (FOSC) contains several phylogenetic lineages. Phylogenetic studies identified two to three major clades within the FOSC. The mitochondrial sequences are highly informative phylogenetic markers, but have been mostly neglected due to technical difficulties.
RESULTS:A total of 61 complete mitogenomes of FOSC strains were de novo assembled and annotated. Length variations and intron patterns support the separation of three phylogenetic species. The variable region of the mitogenome that is typical for the genus Fusarium shows two new variants in the FOSC. The variant typical for Fusarium is found in members of all three clades, while variant 2 is found in clades 2 and 3 and variant 3 only in clade 2. The extended set of loci analyzed using a new implementation of the genealogical concordance species recognition method support the identification of three phylogenetic species within the FOSC. Comparative analysis of the mitogenomes in the FOSC revealed ongoing mitochondrial recombination within, but not between phylogenetic species.
CONCLUSIONS:The recombination indicates the presence of a parasexual cycle in F. oxysporum. The obstacles hindering the usage of the mitogenomes are resolved by using next generation sequencing and selective genome assemblers, such as GRAbB. Complete mitogenome sequences offer a stable basis and reference point for phylogenetic and population genetic studies.
巻・号 18(1)
ページ 735
公開日 2017-9-18
DOI 10.1186/s12864-017-4116-5
PII 10.1186/s12864-017-4116-5
PMID 28923029
PMC PMC5604515
MeSH Conserved Sequence Fusarium / genetics* Genetic Variation Genome, Mitochondrial / genetics* Genomics Introns / genetics Phylogeny Recombination, Genetic*
IF 3.594
引用数 26
リソース情報
一般微生物 JCM 11502