RRC ID 511
Author Martinez L, Almagro JC, Coll JL, Herrera RJ.
Title Sequence variability in the fibroin-H intron of domesticated and wild silk moths.
Journal Insect Biochem. Mol. Biol.
Abstract The single intron of the heavy-chain fibroin gene in domesticated (Bombyx mori) and wild (B. mandarina) silk moths has a length of approximately 1000 nucleotides. It is located only 57 bp from the gene's core promoter and harbors multiple AT-rich regulatory elements that have been found to enhance the basal level of transcription in vitro. In this work, the intronic nucleotide variability among members of both Bombyx species is analyzed. The intron sequences of B. mori strains k120 and Nistari, as well as B. mandarina specimens from Japan and Korea, were obtained. This information was compared with the previously reported sequences of B. mori strains p50 and C-108, as well as an additional B. mandarina specimen collected in Japan. We found a total of 26 variant positions, including variants shared by members of both species and species-specific changes. The potential functional role of these variants was investigated by using the program MatInspector to search for putative binding sites of transcription factors within the intron. We detected a multitude of putative binding elements distributed along the entire intronic sequence. Among them, 22 correspond to protein binding domains that are known to regulate fibroin transcription. The mapping of multiple variant positions within these putative binding sequences as well as in known regulatory elements of the intron argue for functional significance on the regulation of transcription.
Volume 34(4)
Pages 343-52
Published 2004-4
DOI 10.1016/j.ibmb.2003.12.002
PII S0965174803002339
PMID 15041018
MeSH Animals Animals, Domestic Animals, Wild Base Sequence Binding Sites Bombyx / chemistry* Bombyx / genetics* Fibroins / genetics* Fibroins / metabolism Forkhead Transcription Factors Genetic Variation Insect Proteins / genetics* Insect Proteins / metabolism Introns / genetics* Molecular Sequence Data Nuclear Proteins / metabolism Phylogeny Protein Binding Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Alignment Species Specificity Transcription Factors / metabolism
IF 3.618
Times Cited 7
Silkworms p50