RRC ID 51130
著者 Ojima-Kato T, Yamamoto N, Nagai S, Shima K, Akiyama Y, Ota J, Tamura H.
タイトル Application of proteotyping Strain Solution™ ver. 2 software and theoretically calculated mass database in MALDI-TOF MS typing of Salmonella serotype.
ジャーナル Appl Microbiol Biotechnol
Abstract Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based microbial identification is a popular analytical method. Strain Solution proteotyping software available for MALDI-TOF MS has great potential for the precise and detailed discrimination of microorganisms at serotype- or strain-level, beyond the conventional mass fingerprinting approaches. Here, we constructed a theoretically calculated mass database of Salmonella enterica subspecies enterica consisting of 12 biomarker proteins: ribosomal proteins S8, L15, L17, L21, L25, and S7, Mn-cofactor-containing superoxide dismutase (SodA), peptidyl-prolyl cis-trans isomerase C (PPIase C), and protein Gns, and uncharacterized proteins YibT, YaiA, and YciF, that can allow serotyping of Salmonella. Strain Solution ver. 2 software with the novel database constructed in this study demonstrated that 109 strains (94%), including the major outbreak-associated serotypes, Enteritidis, Typhimurium, and Infantis, could be correctly identified from others by colony-directed MALDI-TOF MS using 116 strains belonging to 23 kinds of typed and untyped serotypes of S. enterica from culture collections, patients, and foods. We conclude that Strain Solution ver. 2 software integrated with the accurate mass database will be useful for the bacterial proteotyping by MALDI-TOF MS-based microbial classification in the clinical and food safety fields.
巻・号 101(23-24)
ページ 8557-8569
公開日 2017-12-1
DOI 10.1007/s00253-017-8563-3
PII 10.1007/s00253-017-8563-3
PMID 29032472
MeSH Bacterial Proteins / analysis* Bacterial Proteins / chemistry* Computational Biology / methods* Food Microbiology Humans Salmonella Infections / microbiology Salmonella enterica / chemistry* Salmonella enterica / classification* Salmonella enterica / isolation & purification Serotyping / methods* Software Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
IF 3.53
引用数 7
リソース情報
一般微生物 JCM 3919