論文 - 詳細
| RRC ID | 51130 |
|---|---|
| 著者 | Ojima-Kato T, Yamamoto N, Nagai S, Shima K, Akiyama Y, Ota J, Tamura H. |
| タイトル | Application of proteotyping Strain Solution™ ver. 2 software and theoretically calculated mass database in MALDI-TOF MS typing of Salmonella serotype. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based microbial identification is a popular analytical method. Strain Solution proteotyping software available for MALDI-TOF MS has great potential for the precise and detailed discrimination of microorganisms at serotype- or strain-level, beyond the conventional mass fingerprinting approaches. Here, we constructed a theoretically calculated mass database of Salmonella enterica subspecies enterica consisting of 12 biomarker proteins: ribosomal proteins S8, L15, L17, L21, L25, and S7, Mn-cofactor-containing superoxide dismutase (SodA), peptidyl-prolyl cis-trans isomerase C (PPIase C), and protein Gns, and uncharacterized proteins YibT, YaiA, and YciF, that can allow serotyping of Salmonella. Strain Solution ver. 2 software with the novel database constructed in this study demonstrated that 109 strains (94%), including the major outbreak-associated serotypes, Enteritidis, Typhimurium, and Infantis, could be correctly identified from others by colony-directed MALDI-TOF MS using 116 strains belonging to 23 kinds of typed and untyped serotypes of S. enterica from culture collections, patients, and foods. We conclude that Strain Solution ver. 2 software integrated with the accurate mass database will be useful for the bacterial proteotyping by MALDI-TOF MS-based microbial classification in the clinical and food safety fields. |
| 巻・号 | 101(23-24) |
| ページ | 8557-8569 |
| 公開日 | 2017-12-1 |
| DOI | 10.1007/s00253-017-8563-3 |
| PII | 10.1007/s00253-017-8563-3 |
| PMID | 29032472 |
| MeSH | Bacterial Proteins / analysis* Bacterial Proteins / chemistry* Computational Biology / methods* Food Microbiology Humans Salmonella Infections / microbiology Salmonella enterica / chemistry* Salmonella enterica / classification* Salmonella enterica / isolation & purification Serotyping / methods* Software Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| IF | 3.53 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 3919 |